Parveen Rashida, Asghar Ali, Anjum Faqir M, Khan Muhammad I, Arshad Muhammad Sajid, Yasmeen Ammara
Lipids Health Dis. 2013 Apr 23;12:52. doi: 10.1186/1476-511X-12-52.
The use of bioactive antioxidants in feed of broiler to mitigate reactive oxygen species (ROS) in biological systems is one of promising nutritional strategies. The aim of present study was to alleviate ROS production in mitochondrial fraction (MF) of meat by supplemented dietary antioxidant in feed of broiler. For this purpose, mitochondria specific antioxidant: α-lipoic acid (25 mg, 75 mg and 150 mg) with or without combination of α-tocopherol acetate (200 mg) used in normal and palm olein oxidized oil (4%) supplemented feed. One hundred and eighty one day old broiler birds were randomly divided into six treatments and provided the mentioned feed from third week. Feed intake, feed conversion ratio (FCR) remained statistically same in all groups while body weight decreased in supplemented groups accordingly at the end of study. The broiler meat MF antioxidant potential was significantly improved by feeding supplemented feed estimated as 1,1-di phenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, 2,2-azinobis-(3- ethylbenzothiazoline-6-sulphonic acid) (ABTS+) and thiobarbituric acid reactive substances (TBARS). The maximum antioxidant activity was depicted in group fed on 150 mg/kg α-lipoic acid (ALA) and 200 mg/kg α-tocopherol acetate (ATA) (T4) in both breast and leg MF. Moreover, TBARS were higher in leg as compared to breast MF. Although, oxidized oil containing feed reduced the growth, lipid stability and antioxidant potential of MF whilst these traits were improved by receiving feed containing ALA and ATA. ALA and ATA showed higher deposition in T4 group while least in group received oxidized oil containing feed (T5). Positive correlation exists between DPPH free radical scavenging activity and the ABTS + reducing activity. In conclusion, ALA and ATA supplementation in feed had positive effect on antioxidant status of MF that consequently diminished the oxidative stress in polyunsaturated fatty acid enriched meat.
在肉鸡饲料中使用生物活性抗氧化剂以减轻生物系统中的活性氧（ROS）是一种很有前景的营养策略。本研究的目的是通过在肉鸡饲料中添加膳食抗氧化剂来减轻肉中线粒体部分（MF）的ROS产生。为此，将线粒体特异性抗氧化剂：α-硫辛酸（25毫克、75毫克和150毫克）与或不与醋酸α-生育酚（200毫克）组合，用于正常和添加了4%棕榈油精氧化油的饲料中。180只1日龄的肉鸡被随机分为6组，并从第三周开始提供上述饲料。在研究结束时，所有组的采食量、饲料转化率（FCR）在统计学上保持相同，而添加组的体重相应下降。通过喂食添加饲料，以1,1-二苯基-2-苦基肼（DPPH）自由基清除活性、2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS+）和硫代巴比妥酸反应物质（TBARS）来评估，肉鸡肌肉MF的抗氧化潜力得到显著改善。在胸肌和腿肌MF中，喂食150毫克/千克α-硫辛酸（ALA）和200毫克/千克醋酸α-生育酚（ATA）的组（T4）表现出最大的抗氧化活性。此外，腿肌中的TBARS高于胸肌MF。虽然含氧化油的饲料降低了MF的生长、脂质稳定性和抗氧化潜力，但通过接受含ALA和ATA的饲料，这些特性得到了改善。ALA和ATA在T4组中的沉积量较高，而在接受含氧化油饲料的组（T5）中最少。DPPH自由基清除活性与ABTS+还原活性之间存在正相关。总之，在饲料中添加ALA和ATA对MF的抗氧化状态有积极影响，从而减少了富含多不饱和脂肪酸的肉中的氧化应激。
