Insect Resource Center for Engineering and Technology of Liaoning Province, College of Bioscience and Biotechnology, Shenyang Agricultural University, Liaoning, Shenyang 110866, China.
J Agric Food Chem. 2013 May 15;61(19):4599-605. doi: 10.1021/jf4006092. Epub 2013 May 3.
Two 12 kDa FK506-binding protein (FKBP12) genes were isolated and characterized from Chinese oak silkworm Antheraea pernyi , an important agricultural and edible insect, designated ApFKBP12 A and B, respectively. Both ApFKBP12 A and B contained 108 amino acids with 82% sequence identity. Phylogenetic analysis showed that FKBP12 B sequences of A. pernyi, Bombyx mori , and Danaus plexippus were clearly separated from FKBP12 A sequences of these three species, suggesting that insect FKBP12 A and B may have been evolving independently. RT-PCR analyses revealed that two ApFKBP12 genes were expressed during the four developmental stages and in all tested tissues, and that the mRNA expression level of the ApFKBP12 A gene was significantly higher than that of the ApFKBP12 B gene. After heat shock treatment, expressions of the two FKBP12 genes were up-regulated, but at different time points. The results suggested that each paralogue of the FKBP12 genes may play a distinct functional role in the development of A. pernyi.
从重要的农业和食用昆虫——野桑蚕（Antheraea pernyi）中分离并鉴定了两个 12 kDa FK506 结合蛋白（FKBP12）基因，分别命名为 ApFKBP12 A 和 B。ApFKBP12 A 和 B 均包含 108 个氨基酸，序列同一性为 82%。系统进化分析表明，野桑蚕、家蚕和大蚕的 FKBP12 B 序列与这三个物种的 FKBP12 A 序列明显分离，表明昆虫 FKBP12 A 和 B 可能独立进化。RT-PCR 分析显示，两个 ApFKBP12 基因在四个发育阶段和所有测试的组织中均有表达，并且 ApFKBP12 A 基因的 mRNA 表达水平明显高于 ApFKBP12 B 基因。热休克处理后，两个 FKBP12 基因的表达均上调，但时间点不同。结果表明，FKBP12 基因的每个同源基因在野桑蚕的发育中可能发挥独特的功能作用。
