中国橡树蚕（Antheraea pernyi）两种 12 kDa FK506 结合蛋白基因的分子克隆与特性分析。

Molecular cloning and characterization of two 12 kDa FK506-binding protein genes in the Chinese oak silkworm, Antheraea pernyi.

机构信息

Insect Resource Center for Engineering and Technology of Liaoning Province, College of Bioscience and Biotechnology, Shenyang Agricultural University, Liaoning, Shenyang 110866, China.

出版信息

J Agric Food Chem. 2013 May 15;61(19):4599-605. doi: 10.1021/jf4006092. Epub 2013 May 3.

DOI:10.1021/jf4006092

PMID:23617895

Abstract

Two 12 kDa FK506-binding protein (FKBP12) genes were isolated and characterized from Chinese oak silkworm Antheraea pernyi , an important agricultural and edible insect, designated ApFKBP12 A and B, respectively. Both ApFKBP12 A and B contained 108 amino acids with 82% sequence identity. Phylogenetic analysis showed that FKBP12 B sequences of A. pernyi, Bombyx mori , and Danaus plexippus were clearly separated from FKBP12 A sequences of these three species, suggesting that insect FKBP12 A and B may have been evolving independently. RT-PCR analyses revealed that two ApFKBP12 genes were expressed during the four developmental stages and in all tested tissues, and that the mRNA expression level of the ApFKBP12 A gene was significantly higher than that of the ApFKBP12 B gene. After heat shock treatment, expressions of the two FKBP12 genes were up-regulated, but at different time points. The results suggested that each paralogue of the FKBP12 genes may play a distinct functional role in the development of A. pernyi.

摘要

从重要的农业和食用昆虫——野桑蚕（Antheraea pernyi）中分离并鉴定了两个 12 kDa FK506 结合蛋白（FKBP12）基因，分别命名为 ApFKBP12 A 和 B。ApFKBP12 A 和 B 均包含 108 个氨基酸，序列同一性为 82%。系统进化分析表明，野桑蚕、家蚕和大蚕的 FKBP12 B 序列与这三个物种的 FKBP12 A 序列明显分离，表明昆虫 FKBP12 A 和 B 可能独立进化。RT-PCR 分析显示，两个 ApFKBP12 基因在四个发育阶段和所有测试的组织中均有表达，并且 ApFKBP12 A 基因的 mRNA 表达水平明显高于 ApFKBP12 B 基因。热休克处理后，两个 FKBP12 基因的表达均上调，但时间点不同。结果表明，FKBP12 基因的每个同源基因在野桑蚕的发育中可能发挥独特的功能作用。