State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
Talanta. 2013 May 15;109:147-51. doi: 10.1016/j.talanta.2013.02.006. Epub 2013 Feb 19.
A tubular biofilm reactor (BFR) based online biochemical oxygen demand prototype was applied in Taihu Lake, China. Municipal tap water was used instead of conventional phosphate buffer as blank solution to avoid phosphate pollution. The background organic compounds in municipal tap water were taken into account and they were validated to result in negative deviation to accuracy. The microbial endogenous respiration was experimentally validated to be sensitive to salt ionic strength, and municipal tap water as blank was thought to generate positive deviation to accuracy. The system was continuously operated over 2 months without man intervention, and the automated monitoring data agreed well with that of the conventional BOD5 methods. The BFR resisted the frequent measurements with samples of high turbidity, and the BOD monitoring data indicated the index of biodegradable organic compounds of Taihu Lake was accorded with the second class described in the environmental quality standard of surface water. Analyzed together with permanganate index on site, Taihu Lake was revealed to be of good capacity of self cleaning. Importantly, field application study of new BOD method made it more objective in evaluating its applicability, and could provide practical information and useful improvements in the process of commercializing.
基于管状生物膜反应器（BFR）的在线生化需氧量原型被应用于中国太湖。为避免磷酸盐污染，使用市政自来水代替传统的磷酸盐缓冲液作为空白溶液。考虑到市政自来水中的背景有机化合物，它们被验证会导致准确度产生负偏差。微生物内呼吸被实验证明对盐离子强度敏感，而认为市政自来水作为空白会导致准确度产生正偏差。该系统在无人干预的情况下连续运行了 2 个月，自动监测数据与传统 BOD5 方法吻合较好。BFR 能够抵抗频繁测量高浊度样品的干扰，BOD 监测数据表明太湖可生物降解有机化合物的指标符合地表水环境质量标准中二类的描述。与现场高锰酸盐指数分析相结合，表明太湖具有良好的自净能力。重要的是，新 BOD 方法的现场应用研究使其在评估适用性方面更加客观，并为商业化过程提供了实用信息和有益改进。
