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对两种不同的八孢毛霉（Phycomyces blakesleeanus）突变体进行体外特性分析，这些突变体的八氢番茄红素去饱和作用受损。

In vitro characterization of two different Phycomyces blakesleeanus mutants with impaired phytoene desaturation.

作者信息

Schmidt A, Sandmann G

机构信息

Lehrstuhl für Physiologie und Biochemie der Pflanzen, Universität Konstanz, Federal Republic of Germany.

出版信息

J Bacteriol. 1990 Jul;172(7):4103-5. doi: 10.1128/jb.172.7.4103-4105.1990.

DOI:10.1128/jb.172.7.4103-4105.1990
PMID:2361949
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC213398/
Abstract

In vitro phytoene desaturation was investigated in two Phycomyces blakesleeanus mutants, C5 and S442, in which phytoene is accumulated instead of beta-carotene. For strain C5 but not strain S442 the phenotypic block of phytoene conversion could be overcome in vitro by the addition of Tween 40. Immunodetection of phytoene desaturase revealed in all cases the presence of a 40-kilodalton protein.

摘要

在两种米根霉突变体C5和S442中研究了体外八氢番茄红素去饱和作用，在这两种突变体中积累的是八氢番茄红素而非β-胡萝卜素。对于C5菌株而非S442菌株，通过添加吐温40可在体外克服八氢番茄红素转化的表型阻断。八氢番茄红素去饱和酶的免疫检测在所有情况下均显示存在一种40千道尔顿的蛋白质。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc91/213398/cf5b277c5105/jbacter00121-0580-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc91/213398/cf5b277c5105/jbacter00121-0580-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc91/213398/cf5b277c5105/jbacter00121-0580-a.jpg

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