Frot-Coutaz J, Berthillier G, Got R
FEBS Lett. 1975 Mar 15;52(1):81-4. doi: 10.1016/0014-5793(75)80643-0.
Rat liver microsomal membranes have been shown to contain a biosynthetic pathway of UDP-glucose. In addition, they are able to bind UDP-glucose in a reversible manner, As UDP-glucose is also metabolized in these membranes, the study of the binding has been performed with a microsomal Triton X 100 extract. This reversible binding depends on pH (maximum at pH 8.1) and manganous ions, and disappears at pH 6.5. It exhibits a high affinity (K-diss equals 3 mu M), and a narrow specifity for UDP-glucose. Proteolytic digestion inhibits the binding up to 90%, showing that the UDP-glucose receptor has a proteic nature. These binding characteristics have been also found in the membranes themselves, indicating that the detergent solubilization does not destroy the protein binding capacity.
已证明大鼠肝微粒体膜含有UDP-葡萄糖的生物合成途径。此外，它们能够以可逆方式结合UDP-葡萄糖。由于UDP-葡萄糖也在这些膜中代谢，因此结合研究是用微粒体Triton X 100提取物进行的。这种可逆结合取决于pH值（在pH 8.1时最大）和锰离子，在pH 6.5时消失。它表现出高亲和力（解离常数K-diss等于3 μM），并且对UDP-葡萄糖具有狭窄的特异性。蛋白水解消化可将结合抑制高达90%，表明UDP-葡萄糖受体具有蛋白质性质。在膜本身中也发现了这些结合特性，表明去污剂增溶不会破坏蛋白质结合能力。
