Institute of Molecular Biomedicine, Comenius University, Bratislava, Slovakia; Division of Nephrology, RWTH University, Aachen, Germany.
Biotechnol Adv. 2013 Dec;31(8):1247-59. doi: 10.1016/j.biotechadv.2013.04.004. Epub 2013 Apr 23.
In vivo phage display is a high-throughput method for identifying target ligands specific for different vascular beds. Targeting is possible due to the heterogeneous expression of receptors and other antigens in a particular vascular bed. Such expression is additionally influenced by the physiological or pathological status of the vasculature. In vivo phage display represents a technique that is usable in both, vascular mapping and targeted drug development. In this review, several important methodological aspects of in vivo phage display experiments are discussed. These include choosing an appropriate phage library, an appropriate animal model and the route of phage library administration. In addition, peptides or antibodies identified by in vivo phage display homing to specific types of vascular beds, including the altered vasculature present in several types of diseases are summarized. Still, confirmation in independent experiments and reproduction of identified sequences are needed for enhancing the clinical applicability of in vivo phage display research.
体内噬菌体展示是一种高通量的方法，用于鉴定针对不同血管床的靶标配体。由于特定血管床中受体和其他抗原的异质表达，靶向是可能的。这种表达还受到脉管系统生理或病理状态的影响。体内噬菌体展示代表了一种可用于血管绘图和靶向药物开发的技术。在这篇综述中，讨论了体内噬菌体展示实验的几个重要方法学方面。这些方面包括选择合适的噬菌体文库、合适的动物模型和噬菌体文库给药途径。此外，还总结了通过体内噬菌体展示鉴定出的靶向特定类型血管床的肽或抗体，包括几种疾病中存在的改变的脉管系统。然而，需要通过独立实验进行验证并重复鉴定出的序列，以增强体内噬菌体展示研究的临床适用性。
