State Key Laboratory of Biotherapy and Cancer Center, Department of Neurosurgery, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, PR China.
Int J Biol Macromol. 2013 Aug;59:227-34. doi: 10.1016/j.ijbiomac.2013.04.051. Epub 2013 Apr 23.
In an effort to decrease the aggregation of tetracalcium phosphate (TTCP, Ca4(PO4)2O) in composites and develop better bone substitute materials, a series of poly(l-lactic acid) (PLLA)-grafted TTCP (g-TTCP) particles were prepared by a ring-opening polymerization with l-lactide (the monomer for synthesizing PLLA) in the presence of catalyst stannous octoate [Sn(Oct)2]. The g-TTCP/poly(1,4-butylene succinate) (PBS) composites with the different g-TTCP contents were prepared via melting processing. The bonding between the PLLA and the TTCP particles was analyzed by FTIR, TG, (1)H NMR and XPS. The results confirmed that the PLLA was grafted on the surface of the TTCP particles. Time-dependent phase monitoring indicated that the g-TTCP had enhanced dispersion in the PBS solution. Water contact angle measurement and cell culture were also used to investigate the properties of the g-TTCP/PBS composites. The g-TTCP in composites provided more favorable environments for rat osteoblast to attach and grow on the surface of the g-TTCP/PBS composites. Cell proliferated well in the extracted solution of the g-TTCP/PBS composites with different g-TTCP content, and there was no necrotic or suspended cells appeared.
为了减少四钙磷酸盐（TTCP，Ca4(PO4)2O）在复合材料中的聚集并开发更好的骨替代材料，通过在催化剂辛酸亚锡[Sn(Oct)2]存在下用内消旋丙交酯（合成 PLLA 的单体）开环聚合制备了一系列聚（L-乳酸）（PLLA）接枝 TTCP（g-TTCP）颗粒。通过熔融加工制备了具有不同 g-TTCP 含量的 g-TTCP/聚（1,4-丁二酸丁二醇酯）（PBS）复合材料。通过 FTIR、TG、（1）H NMR 和 XPS 分析了 PLLA 和 TTCP 颗粒之间的键合。结果证实 PLLA 接枝在 TTCP 颗粒的表面上。时相监测表明 g-TTCP 在 PBS 溶液中具有增强的分散性。还通过水接触角测量和细胞培养来研究 g-TTCP/PBS 复合材料的性能。复合材料中的 g-TTCP 为大鼠成骨细胞在 g-TTCP/PBS 复合材料表面附着和生长提供了更有利的环境。细胞在不同 g-TTCP 含量的 g-TTCP/PBS 复合材料提取溶液中增殖良好，没有出现坏死或悬浮细胞。
