Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada, Kobe 657-8501, Japan.
N Biotechnol. 2013 Jun 25;30(5):531-5. doi: 10.1016/j.nbt.2013.04.005. Epub 2013 Apr 26.
The present study reports the design of a novel bioanode to directly utilize starch as a fuel in an enzymatic biofuel cell. The enzymatic fuel cell is based on three enzymes (alpha-amylase, glucoamylase and glucose oxidase). The carbon paste electrode containing these three enzymes and tetrathiafulvalene can both saccharize and oxidize starchy biomass. In cyclic voltammetry, catalytic currents were successfully observed with both glucose and starchy white rice used as a substrate. Finally, a membrane-less white rice/O2 biofuel cell was assembled and the electrochemical performance was evaluated. The three enzyme based electrode was used as a bioanode and an immobilized bilirubin oxidase (derived from Myrothecium verrucaria) electrode was used as a biocathode. The biofuel cell delivered an open circuit voltage of 0.522V and power density of up to 99.0 μWcm(-2). Our results show that a readily available fuel can be used for enzymatic fuel cells, and will lead to new designs.
本研究报告了一种新型生物阳极的设计，该生物阳极可直接将淀粉用作酶生物燃料电池中的燃料。该酶燃料电池基于三种酶（α-淀粉酶、糖化酶和葡萄糖氧化酶）。含有这三种酶和四硫富瓦烯的碳糊电极既可以糖化也可以氧化淀粉生物质。在循环伏安法中，成功地观察到以葡萄糖和淀粉白米为底物的催化电流。最后，组装了无膜白米/O2 生物燃料电池，并对其电化学性能进行了评估。基于三种酶的电极用作生物阳极，固定化胆红素氧化酶（来源于 Myrothecium verrucaria）电极用作生物阴极。该生物燃料电池的开路电压为 0.522V，最大功率密度高达 99.0 μWcm(-2)。我们的结果表明，一种现成的燃料可用于酶燃料电池，并将导致新的设计。
