Wang Xiaoyuan
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Wei Sheng Wu Xue Bao. 2013 Feb 4;53(2):111-7.
In most Gram-negative bacteria, the Kdo2-lipid A moiety of lipopolysaccharide forms the outer monolayer of the outer membrane. Diverse covalent modifications of Kdo2-lipid A may occur in bacteria to survive different environmental conditions. Kdo2-lipid A is usually synthesized on the cytoplasmic surface of the inner membrane. After the core oligosaccharide is attached to Kdo2-lipid A, the molecule is flipped to the outer surface of the inner membrane, where the O-antigen repeats are attached to form lipopolysaccharide. Kdo2-lipid A could activate the innate immune system through TLR4. Therefore, researches on the structure modification of Kdo2-lipid A would be useful for developing new vaccines and adjuvants.
在大多数革兰氏阴性菌中，脂多糖的Kdo2-脂质A部分构成外膜的外单层。细菌中Kdo2-脂质A可能会发生多种共价修饰，以在不同环境条件下生存。Kdo2-脂质A通常在内膜的细胞质表面合成。核心寡糖连接到Kdo2-脂质A后，该分子翻转到内膜的外表面，在那里O抗原重复序列连接形成脂多糖。Kdo2-脂质A可通过TLR4激活先天免疫系统。因此，对Kdo2-脂质A结构修饰的研究将有助于开发新型疫苗和佐剂。
