Janssen D, De Rycke P H, De Ridder L
Laboratory of Zoophysiology, State University of Gent, Belgium.
Parasitology. 1990 Jun;100 Pt 3:453-8. doi: 10.1017/s0031182000078756.
Vesiculated protoscoleces (VP) were produced by culturing freshly collected protoscoleces from Echinococcus granulosus horse liver hydatids in RPMI 1640 monophasic medium at 37 degrees C for 18 days. Half of the VP were used as such, the other half used after killing them by freeze-thawing. Nine-day-old chicken heart fragments (CHF) were cultured in MEM at 37 degrees C for 72 h. Subsequently, CHF were put together with live and dead VP, respectively, for up to 53 days, on a semisolid medium consisting of agar, Ringer's and MEM. Time-dependent histological observations revealed that dead VP were surrounded by CHF cells. Dead VP tissue was eventually internalized and disintegrated in about 1 week. Live VP penetrated into the CHF tissue and further developed into small hydatid cysts, located within the boundaries of the experimental 'host' tissue. The amorphous-looking contact region PAP-stained positively only with anti-E. granulosus serum and not with anti-CHF serum; it was considered identical to the normal laminated layer. The invasion of VP in CHF tissue proved to be different from a tumour or a bacterial invasion: it was concluded that the confrontation of VP and CHF had resulted in an 'in vitro cohabitation' rather than in an 'in vitro infection'.
泡状原头节（VP）是通过将刚从细粒棘球绦虫马肝包虫中收集的原头节在RPMI 1640单相培养基中于37℃培养18天产生的。一半的VP直接使用，另一半经冻融杀死后使用。将9日龄鸡心片段（CHF）在MEM中于37℃培养72小时。随后，分别将CHF与活的和死的VP放在一起，在由琼脂、林格氏液和MEM组成的半固体培养基上培养长达53天。随时间变化的组织学观察表明，死的VP被CHF细胞包围。死的VP组织最终在大约1周内被内化并分解。活的VP侵入CHF组织并进一步发育成小的包虫囊肿，位于实验“宿主”组织的边界内。外观无定形的接触区域用PAP染色后仅与抗细粒棘球绦虫血清呈阳性反应，而与抗CHF血清不发生反应；它被认为与正常的板层相同。VP在CHF组织中的侵入被证明不同于肿瘤或细菌的侵入：得出的结论是，VP与CHF的对峙导致了“体外共生”而非“体外感染”。
