使用Cobe Spectra细胞分离器进行骨髓移植处理。

Bone marrow processing for transplantation using Cobe Spectra cell separator.

作者信息

Veljković Dobrila, Nonković Olivera Šerbić, Radonjić Zorica, Kuzmanović Miloš, Zečević Zeljko

机构信息

Department of Transfusion Medicine, Institute for Mother and Child Health Care of Serbia, Dr. Vukan Čupić, Belgrade, Serbia.

出版信息

Transfus Apher Sci. 2013 Jun;48(3):359-63. doi: 10.1016/j.transci.2013.04.017. Epub 2013 Apr 28.

DOI:10.1016/j.transci.2013.04.017

PMID:23628356

Abstract

Concentration of bone marrow aspirates is an important prerequisite prior to infusion of ABO incompatible allogeneic marrow and prior to cryopreservation and storage of autologous marrow. In this paper we present our experience in processing 15 harvested bone marrow for ABO incompatible allogeneic and autologous bone marrow (BM) transplantation using Cobe Spectra® cell separator. BM processing resulted in the median recovery of 91.5% CD34+ cells, erythrocyte depletion of 91% and volume reduction of 81%. BM processing using cell separator is safe and effective technique providing high rate of erythrocyte depletion and volume reduction, and acceptable recovery of the CD34+ cells.

摘要

骨髓抽吸物的浓缩是输注ABO血型不相合的异基因骨髓之前以及自体骨髓冷冻保存和储存之前的重要前提条件。在本文中，我们介绍了使用Cobe Spectra®细胞分离器处理15份采集的骨髓用于ABO血型不相合的异基因和自体骨髓移植的经验。骨髓处理导致CD34+细胞的中位回收率为91.5%，红细胞清除率为91%，体积减少81%。使用细胞分离器进行骨髓处理是一种安全有效的技术，可实现高红细胞清除率和体积减少率，以及可接受的CD34+细胞回收率。