Hu Xianwen, Zhang Ye, Li Weiyan, Liu Jian, Li Yun
Department of Anesthesiology, The Second Hospital of Anhui Medical University, Hefei, Anhui Province 230601, PR China.
Int J Dev Neurosci. 2013 Aug;31(5):328-33. doi: 10.1016/j.ijdevneu.2013.04.004. Epub 2013 Apr 28.
Previous studies have demonstrated that sevoflurane could attenuate cerebral neuron necrosis and apoptosis in ischemia-reperfusion models in rats. The aim of our study was to investigate the effect of preconditioning with sevoflurane on spatial learning and memory ability after focal cerebral ischemia-reperfusion injury in rats and its potential mechanisms. Focal cerebral ischemia was performed via 1h of middle cerebral artery occlusion (MCAO) followed by reperfusion. Before ischemia, rats were subjected to preconditioning with inhalation of 2.4% sevoflurane for 1h. The spatial learning and memory ability of rats was measured by the Morris water maze. The activity of choline acetyltransferase (ChAT) in hippocampus CA1 region was observed by immunohistochemistry method. We found MCAO elicited a significant decrease of the ability of spatial learning and memory in contrast to the sham surgery controls. However, preconditioning with sevoflurane resulted in significantly ameliorates spatial learning and memory deficit induced by MCAO. Furthermore, the number of ChAT positive cells in hippocampus CA1 region in sevoflurane preconditioning group was striking more than that of ischemia-reperfusion group. All results suggested that preconditioning with 2.4% sevoflurane could ameliorate the ability of spatial learning and memory after focal cerebral ischemia-reperfusion in rats via protecting the cholinergic neurons in hippocampal CA1 region.
先前的研究表明，七氟醚可减轻大鼠缺血再灌注模型中的脑神经元坏死和凋亡。我们研究的目的是探讨七氟醚预处理对大鼠局灶性脑缺血再灌注损伤后空间学习和记忆能力的影响及其潜在机制。通过大脑中动脉闭塞（MCAO）1小时后再灌注来造成局灶性脑缺血。在缺血前，让大鼠吸入2.4%七氟醚预处理1小时。通过莫里斯水迷宫测量大鼠的空间学习和记忆能力。采用免疫组化法观察海马CA1区胆碱乙酰转移酶（ChAT）的活性。我们发现，与假手术对照组相比，MCAO导致空间学习和记忆能力显著下降。然而，七氟醚预处理可显著改善MCAO所致的空间学习和记忆缺陷。此外，七氟醚预处理组海马CA1区ChAT阳性细胞数量明显多于缺血再灌注组。所有结果表明，2.4%七氟醚预处理可通过保护海马CA1区胆碱能神经元来改善大鼠局灶性脑缺血再灌注后的空间学习和记忆能力。
