Department of Laboratory Medicine, Laboratory of Clinical Chemistry, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands.
Clin Biochem. 2013 Sep;46(13-14):1252-6. doi: 10.1016/j.clinbiochem.2013.04.014. Epub 2013 Apr 26.
While urine sampling is necessary in the diagnosis of urinary tract infection and electrolyte disturbances, the collection of urine in neonates and non-toilet-trained children is often difficult. A universal urine collection method providing representative urinalyses results is needed. The objective of this study is to evaluate the applicability of the currently used urine collection pads (gauze compresses) and a new urine collection device (Peespot).
We tested the reliability of routine (semi-)quantitative urinalysis results with these two different kinds of urine collection methods in a laboratory model. Although important in clinical diagnosis, we did not evaluate the effects on cellular and other components such as casts in the urinary sediment.
Most semi-quantitative variables determined by urine stick (pH, blood, protein, leukocytes, nitrite, glucose, ketones, bilirubin and urobilinogen) gave concordant results for both methods compared with native urine. Using the Peespot urine collection device, reliable quantitative results were obtained for calcium, chloride, glucose, magnesium, phosphate, potassium, sodium, osmolality, urea nitrogen and urate compared with native urine. Data were concordant only for chloride, phosphate, glucose, sodium and urea nitrogen by use of gauze compresses.
Urine collection pads are non-invasive methods useful in the collection of urine in non-toilet-trained children. Because of better practical standardisation and more reliable (semi-) quantitative urinalysis results, the Peespot urine collection device is preferred for the collection of urine.
虽然尿液采样在尿路感染和电解质紊乱的诊断中是必要的,但在新生儿和非如厕训练儿童中收集尿液通常很困难。需要一种能够提供代表性尿液分析结果的通用尿液收集方法。本研究的目的是评估目前使用的尿液收集垫(纱布压缩垫)和一种新的尿液收集装置(Peespot)的适用性。
我们在实验室模型中测试了这两种不同的尿液收集方法对常规(半)定量尿液分析结果的可靠性。虽然在临床诊断中很重要,但我们没有评估其对尿液沉淀物中的细胞和其他成分(如管型)的影响。
与原生尿液相比,尿液棒(pH 值、血液、蛋白质、白细胞、亚硝酸盐、葡萄糖、酮体、胆红素和尿胆素原)测定的大多数半定量变量与两种方法均一致。使用 Peespot 尿液收集装置,与原生尿液相比,可获得钙、氯、葡萄糖、镁、磷酸盐、钾、钠、渗透压、尿素氮和尿酸的可靠定量结果。仅使用纱布压缩垫时,数据仅在氯、磷、葡萄糖、钠和尿素氮方面一致。
尿液收集垫是非侵入性方法,可用于收集非如厕训练儿童的尿液。由于更好的实用标准化和更可靠的(半)定量尿液分析结果,Peespot 尿液收集装置更适合用于收集尿液。