Johns Hopkins Physical Sciences - Oncology Center, The Johns Hopkins University, Baltimore, MD, USA.
Cell Adh Migr. 2013 May-Jun;7(3):293-6. doi: 10.4161/cam.24804. Epub 2013 Apr 29.
Efficient cell migration is central to the normal development of tissues and organs and is involved in a wide range of human diseases, including cancer metastasis, immune responses, and cardiovascular disorders. Mesenchymal migration is modulated by focal-adhesion proteins, which organize into large integrin-rich protein complexes at the basal surface of adherent cells. Whether the extent of clustering of focal-adhesion proteins is actually required for effective migration is unclear. We recently demonstrated that the depletion of major focal-adhesion proteins, as well as modulation of matrix compliance, actin assembly, mitochondrial activity, and DNA recombination, all converged into highly predictable, inter-related, biphasic changes in focal adhesion size and cell migration. Herein, we further discuss the role of focal adhesions in controlling cell spreading and test their potential role in cell migration.
细胞的高效迁移对于组织和器官的正常发育至关重要，并且与多种人类疾病有关，包括癌症转移、免疫反应和心血管疾病。细胞间黏附是由黏着斑蛋白调节的，黏着斑蛋白在贴壁细胞的基底表面形成大的整合素丰富的蛋白复合物。黏着斑蛋白的聚集程度是否确实对有效的迁移是必需的，目前还不清楚。我们最近证明，主要黏着斑蛋白的耗竭以及基质顺应性、肌动蛋白组装、线粒体活性和 DNA 重组的调节，都汇聚成黏着斑大小和细胞迁移的高度可预测的、相互关联的、双相变化。在此，我们进一步讨论了黏着斑在控制细胞铺展中的作用，并测试了它们在细胞迁移中的潜在作用。
