Ozeki K, Hoshino T, Aoki H, Masuzawa T
Department of Mechanical Engineering, Ibaraki University, Hitachi, Ibaraki, Japan.
Biomed Mater Eng. 2013;23(3):239-47. doi: 10.3233/BME-130747.
The adsorption behavior of albumin (BSA) and lysozyme (LSZ) on rod-shaped and plate-shaped hydroxyapatite (HA) was investigated to evaluate the influence of crystal orientation and morphology on the selective protein adsorption of HA. The rod-shaped HA was prepared by hydrothermal treatment from β-tricalcium phosphate (β-TCP) in H3PO4 solution (pH 2.0 and 4.0 for HA-pH 2.0 and HA-pH 4.0). The plate-shaped HA was synthesized by hydrolysis of CaHPO4-2H2O (DCPD) in NaOH solution at 40°C and 80°C (HA-40°C and HA-80°C). The synthesized HA was characterized using scanning electron microscopy (SEM) and X-ray diffractometry (XRD). HA-pH 2.0 and HA-pH 4.0 produced rod-shaped crystals that were highly oriented to the a-face plane, whereas HA-40°C and HA-80°C showed a plate-like shape and a c-face preferred orientation. The peak intensity ratio I(300)/I(002) (a/c intensity ratio) from the XRD patterns increased in the following order: HA-80°C, HA-40°C, HA-pH 2.0 and HA-pH 4.0. It also increased as the Ca/P ratio decreased. The amount of adsorbed BSA increased in the following order: HA-pH 4.0, HA-pH 2.0, HA-40°C and HA-80°C. The amount of adsorbed LSZ on HA increased in the following order--HA-pH 2.0, HA-pH 4.0, HA-40°C and HA-80°C--with a corresponding decrease in the a/c intensity ratio. The BSA/LSA adsorption ratio increased with the a/c intensity ratio in the range of 3.3-8.9, and the BSA and LSZ were selectively adsorbed on HA, depending on the crystal shape.
研究了白蛋白（BSA）和溶菌酶（LSZ）在棒状和板状羟基磷灰石（HA）上的吸附行为，以评估晶体取向和形态对HA选择性蛋白质吸附的影响。棒状HA通过在H3PO4溶液（HA-pH 2.0和HA-pH 4.0时pH分别为2.0和4.0）中对β-磷酸三钙（β-TCP）进行水热处理制备。板状HA通过在40°C和80°C的NaOH溶液中水解CaHPO4·2H2O（DCPD）合成（HA-40°C和HA-80°C）。使用扫描电子显微镜（SEM）和X射线衍射仪（XRD）对合成的HA进行表征。HA-pH 2.0和HA-pH 4.0产生高度取向于a面的棒状晶体，而HA-40°C和HA-80°C呈现板状形状且优先取向于c面。XRD图谱中的峰强度比I(300)/I(002)（a/c强度比）按以下顺序增加：HA-80°C、HA-40°C、HA-pH 2.0和HA-pH 4.0。它也随着Ca/P比的降低而增加。吸附的BSA量按以下顺序增加：HA-pH 4.0、HA-pH 2.0、HA-40°C和HA-80°C。HA上吸附的LSZ量按以下顺序增加——HA-pH 2.0、HA-pH 4.0、HA-40°C和HA-80°C——同时a/c强度比相应降低。在3.3-8.9范围内，BSA/LSA吸附比随a/c强度比增加，并且BSA和LSZ根据晶体形状选择性地吸附在HA上。
