Abdel-Rehim Abbi, Abdel-Rehim Mohamed
Department of Analytical Chemistry, Stockholm University, SE10691 Stockholm, Sweden.
Biomed Chromatogr. 2013 Sep;27(9):1188-91. doi: 10.1002/bmc.2925. Epub 2013 Apr 30.
This study presents a new method for collecting and handling saliva samples using an automated analytical microsyringe and microextraction by packed syringe (MEPS). The screening and determination of lidocaine in human saliva samples utilizing MEPS and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were carried out. An exact volume of saliva could be collected. The MEPS C8 -cartridge could be used for 50 extractions before it was discarded. The extraction recovery was about 60%. The pharmacokinetic curve of lidocaine in saliva using MEPS-LC-MS/MS is reported.
本研究提出了一种使用自动分析微量注射器和填充注射器微萃取（MEPS）来收集和处理唾液样本的新方法。利用MEPS和液相色谱-串联质谱法（LC-MS/MS）对人唾液样本中的利多卡因进行了筛查和测定。可以收集精确体积的唾液。MEPS C8柱在丢弃前可用于50次萃取。萃取回收率约为60%。报道了使用MEPS-LC-MS/MS测定唾液中利多卡因的药代动力学曲线。
