Said Rana, Kamel Mohamed, El-Beqqali Aziza, Abdel-Rehim Mohamed
Department of Clinical Pharmacology, Huddinge University Hospital, 17176 Stockholm, Sweden.
Bioanalysis. 2010 Feb;2(2):197-205. doi: 10.4155/bio.09.187.
Microextraction by packed sorbent (MEPS) is used as an online sample-preparation method. The determination of local anesthetics lidocaine, ropivacaine and bupivacaine directly in human blood was performed using MEPS online with LC-MS/MS.
The range of the calibration curves in whole blood was 10-10000 nmol/l. The lower limit of quantification was set to 10.0 nmol/l. The accuracy of the quality control samples ranged from 85 to 97%. The interday precision of the studied analytes was within the range 1-5%. The regression correlation coefficient (r(2)) was over 0.995 for all runs. The present method is rapid, reliable and robust and may be used for therapeutic drug monitoring of studied analytes in whole blood.
This assay allows the analysis of drugs in human blood directly. Sample preparation is simple and automated. The assay reduced the handling time and the cost, and could handle small volumes of whole blood samples (25 µl).
填充吸附剂微萃取（MEPS）用作在线样品制备方法。采用MEPS在线结合液相色谱-串联质谱法直接测定人血中的局麻药利多卡因、罗哌卡因和布比卡因。
全血校准曲线范围为10 - 10000 nmol/L。定量下限设定为10.0 nmol/L。质量控制样品的准确度在85%至97%之间。所研究分析物的日间精密度在1%至5%范围内。所有运行的回归相关系数（r²）均超过0.995。本方法快速、可靠且稳健，可用于全血中所研究分析物的治疗药物监测。
该测定法可直接分析人血中的药物。样品制备简单且自动化。该测定法减少了处理时间和成本，并且能够处理少量全血样品（25 μl）。
