Instituto de Pesquisas Energéticas e Nucleares, IPEN-CNEN/SP, Av. Prof. Lineu Prestes 2242, São Paulo, Brasil.
Microsc Res Tech. 2013 Jul;76(7):704-13. doi: 10.1002/jemt.22221. Epub 2013 Apr 30.
This study evaluated the synergy of professional acidulated fluoride gel (APF) or fluoridated dentifrice application combined with Er,Cr:YSGG laser irradiation on the formation of CaF2 -like products (CaF2 ), in vitro. Thus, 272 bovine enamel slabs were randomly distributed among eight groups: G1: untreated enamel; G2: treated with fluoridated dentifrice (NaF, 1,100 μgF/g); G3: treated with acidulated phosphate fluoride gel (APF, 1.23% F(-) ); G4: irradiated with Er,Cr:YSGG laser at 8.5 J/cm(2) ; G5 and G6: combination of pre-irradiation with Er,Cr:YSGG followed by dentifrice or APF application, respectively; G7: combination of dentifrice application followed by Er,Cr:YSGG irradiation; G8: combination of APF application followed by Er,Cr:YSGG irradiation. After treatments, samples were evaluated by scanning electron microscopy, and the content of CaF2 was determined by an ion specific electrode. Both APF and dentifrice application promoted the formation of CaF2 on enamel, whereas Er,Cr:YSGG irradiation promoted an increase of roughness of the enamel, increasing the surface area. Laser irradiation before fluoridated products increased the content of CaF2 formed when compared to groups that APF or dentifrice were applied isolated. However, the content of CaF2 formed when irradiation was performed after APF or dentifrice was not statically significant when compared to the control groups. In conclusion, Er,Cr:YSGG laser increases the formation of CaF2 on enamel when the irradiation is performed before the application of APF or dentifrice. The association of laser with APF is most promissory for caries prevention because of the higher concentration of CaF2 formation and also the chemical changes promoted by laser irradiation demonstrated in literature.
本研究评估了专业酸性氟化物凝胶（APF）或含氟牙膏与 Er,Cr:YSGG 激光联合应用对 CaF2 样产物（CaF2）形成的协同作用，体外。因此，272 块牛牙釉质板随机分布在 8 个组中：G1：未处理牙釉质；G2：用含氟牙膏（NaF，1100μgF/g）处理；G3：用酸性磷酸盐氟化物凝胶（APF，1.23% F(-)）处理；G4：用 Er,Cr:YSGG 激光照射 8.5 J/cm(2)；G5 和 G6：分别用 Er,Cr:YSGG 激光预先照射后再用牙膏或 APF 处理；G7：牙膏处理后用 Er,Cr:YSGG 激光照射；G8：APF 处理后用 Er,Cr:YSGG 激光照射。处理后，用扫描电子显微镜评估样品，用离子选择性电极测定 CaF2 的含量。APF 和牙膏的应用都促进了牙釉质上 CaF2 的形成，而 Er,Cr:YSGG 激光照射则促进了牙釉质粗糙度的增加，增加了表面积。与单独应用氟化物产品相比，激光照射在前，氟化物产品应用在后，增加了形成的 CaF2 含量。然而，与对照组相比，APF 或牙膏应用后进行激光照射形成的 CaF2 含量并无统计学意义。结论：当 Er,Cr:YSGG 激光照射在前，APF 或牙膏应用在后时，牙釉质上 CaF2 的形成增加。激光与 APF 的联合应用对预防龋齿最有希望，因为形成 CaF2 的浓度更高，而且激光照射所促进的化学变化在文献中已有报道。
