Tian Kangming, Zhou Li, Chen Xianzhong, Shen Wei, Shi Guiyang, Singh Suren, Lu Fuping, Wang Zhengxiang
School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.
Sheng Wu Gong Cheng Xue Bao. 2013 Jan;29(1):111-4.
Glycerol from oil hydrolysis industry is being considered as one of the abundent raw materials for fermentation industry. In present study, the aerobic and anaerobic metabolism and growth properties on glycerol by Esherichia coli CICIM B0013-070, a D-lactate over-producing strain constructed previously, at different temperatures were investigated, followed by a novel fermentation process, named temperature-switched process, was established for D-lactate production from glycerol. Under the optimal condition, lactate yield was increased from 64.0% to 82.6%. Subsequently, the yield of D-lactate from glycerol was reached up to 88.9% while a thermo-inducible promoter was used to regulate D-lactate dehydrogenase transcription.
来自油脂水解工业的甘油正被视为发酵工业丰富的原材料之一。在本研究中，对先前构建的高产D-乳酸菌株大肠杆菌CICIM B0013-070在不同温度下以甘油为底物的好氧和厌氧代谢及生长特性进行了研究，随后建立了一种名为温度切换工艺的新型发酵工艺用于从甘油生产D-乳酸。在最佳条件下，乳酸产量从64.0%提高到了82.6%。随后，当使用热诱导启动子调控D-乳酸脱氢酶转录时，甘油生产D-乳酸的产量达到了88.9%。
