Pfueller S L, Bilston R A, Jane S, Gibson J
Department of Medicine, Monash University Medical School, Alfred Hospital, Prahran, Victoria, Australia.
Thromb Haemost. 1990 Apr 12;63(2):279-81.
In quinine- and quinidine-induced thrombocytopenic purpura IgG antibodies are known to react in a drug-dependent manner with different combinations of surface glycoproteins (GP) Ib, IIb, IIIa and IX. Because endothelial cells share a number of properties of platelets, including the presence of GP IIIa and GPIb-like proteins, we have compared these two cell types for their quinine-dependent IgG binding abilities. By immunoblotting of endothelial cells, quinine-dependent IgG binding from four patient sera was observed only to a 93 kDa component corresponding to GP IIIa. Strong IgG binding independent of the drug was found at 170-180 kDa. Thus endothelial cells express the GP IIIa quinine-dependent epitope on platelet GP IIIa, but not those on other platelet glycoproteins.
在奎宁和奎尼丁诱导的血小板减少性紫癜中，已知IgG抗体以药物依赖的方式与表面糖蛋白（GP）Ib、IIb、IIIa和IX的不同组合发生反应。由于内皮细胞具有许多血小板的特性，包括GP IIIa和GPIb样蛋白的存在，我们比较了这两种细胞类型的奎宁依赖性IgG结合能力。通过对内皮细胞进行免疫印迹分析，仅在与GP IIIa相对应的93 kDa成分上观察到来自四名患者血清的奎宁依赖性IgG结合。在170 - 180 kDa处发现了与药物无关的强IgG结合。因此，内皮细胞在血小板GP IIIa上表达GP IIIa奎宁依赖性表位，但在其他血小板糖蛋白上不表达。
