Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi - 221005, India.
Nanomedicine (Lond). 2014 Mar;9(3):427-40. doi: 10.2217/nnm.13.23. Epub 2013 Apr 30.
Nanodiamonds (NDs) have been evaluated for a wide range of biomedical applications. Thus, thorough investigation of the biocompatibility of NDs has become a research priority. Platelets are highly sensitive and are one of the most abundant cell types found in blood. They have a central role in hemostasis and arterial thrombosis. In this study, we aim to investigate the direct and acute effects of carboxylated NDs on platelet function.
In this study, pro-coagulant parameters such as platelet aggregability, intracellular Ca(2+) flux, mitochondrial transmembrane potential (ΔΨm), generation of reactive oxygen species, surface exposure of phosphatidylserine, electron microscopy, cell viability assay and in vivo thromboembolism were analyzed in great detail.
Carboxylated NDs evoked significant activation of human platelets. When administered intravenously in mice, NDs were found to induce widespread pulmonary thromboembolism, indicating the remarkable thrombogenic potential of this nanomaterial.
Our findings raise concerns regarding the putative biomedical applications of NDs pertaining to diagnostics and therapeutics, and their toxicity and prothrombotic properties should be critically evaluated.
纳米金刚石（NDs）已被评估用于广泛的生物医学应用。因此，对 NDs 的生物相容性进行彻底研究已成为研究重点。血小板非常敏感，是血液中最丰富的细胞类型之一。它们在止血和动脉血栓形成中起核心作用。在这项研究中，我们旨在研究羧基化 NDs 对血小板功能的直接和急性影响。
在这项研究中，详细分析了促凝参数，如血小板聚集性、细胞内 Ca(2+)流、线粒体跨膜电位（ΔΨm）、活性氧的产生、血小板表面磷脂酰丝氨酸的暴露、电子显微镜、细胞活力测定和体内血栓栓塞。
羧基化 NDs 可引起人血小板的显著激活。当静脉内给予小鼠时，发现 NDs 可诱导广泛的肺血栓栓塞，表明这种纳米材料具有显著的促血栓形成潜力。
我们的研究结果引起了人们对 NDs 在诊断和治疗方面的潜在生物医学应用的关注，其毒性和促血栓形成特性应受到严格评估。
