三磷酸腺苷诱导用曲拉通X-100提取的哺乳动物精子中细丝的运动和滑动。
Adenosine triphosphate-induced motility and sliding of filaments in mammalian sperm extracted with Triton X-100.
作者信息
Lindemann C B, Gibbons I R
出版信息
J Cell Biol. 1975 Apr;65(1):147-62. doi: 10.1083/jcb.65.1.147.
Abstract
Bull sperm that had been extracted with 0.2% Triton X-100 could be reactivated with ATP, and their movement closely resembled the motion of intact live sperm. Their motility required the presence of ATP, magnesium, and a medium of suitable salt concentration and pH. When Triton-extracted bull sperm were digested breifly with trypsin at pH 9.0, they appeared to reatin most of their normal structure, but subsequent exposure of the digested sperm to ATP caused a disintegration by light microscopy, using dark-field illumination, combined with an electron microscope study of preparations of the disintegrated sperm, demonstrated the presence of an active sliding mechanism of filament interaction in bull spermatozoa. Human sperm subjected to the same procedures showed similar patterns of reactivation and of disintegration.
摘要
用0.2% Triton X-100提取的公牛精子可以用ATP重新激活，其运动与完整活精子的运动非常相似。它们的活力需要ATP、镁以及合适盐浓度和pH值的介质。当用胰蛋白酶在pH 9.0下短暂消化经Triton提取的公牛精子时，它们似乎保留了大部分正常结构，但随后将消化后的精子暴露于ATP会导致其在光学显微镜下解体，利用暗视野照明结合对解体精子制剂的电子显微镜研究表明，公牛精子中存在细丝相互作用的活跃滑动机制。经过相同程序处理的人类精子表现出相似的重新激活和解体模式。
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