miR-224 的上调与人类宫颈癌的侵袭性进展和不良预后相关。
Upregulation of microRNA-224 is associated with aggressive progression and poor prognosis in human cervical cancer.
机构信息
Department of Gynaecology and Obstetrics, The Third Affiliated Hospital of Inner Mongolia Medical University, Baotou 014010, China.
出版信息
Diagn Pathol. 2013 Apr 30;8:69. doi: 10.1186/1746-1596-8-69.
OBJECTIVE
Accumulating evidence for differential expression of microRNA-224 (miR-224) in various types of human cancer suggests that it may be play a crucial role in tumor biology. The previous microarray detection also shown that miR-224 was one of miRNAs with significant upregulation in cervical cancer tissues relative to adjacent normal tissues. However, little is known about the function of miR-224 in human cervical cancer. The aim of this study was to investigate the clinical significance of miR-224 expression in cervical cancer.
METHODS
MiR-224 expression in 126 pairs of fresh human cervical cancer and adjacent normal tissues was measured by real-time quantitative RT-PCR assay.
RESULTS
miR-224 expression was significantly upregulated in cervical cancer tissues when compared with corresponding adjacent normal tissues (P<0.001). It was also significantly higher in the cancerous tissues of patients with advanced FIGO stage cervical cancer than those with early FIGO stage (P=0.02). In addition, miR-224 was expressed at significantly higher levels in lymph node metastasis-positive patients than in lymph node metastasis-negative patients (P=0.008). Moreover, we found that lesser differentiated tumors expressed higher miR-224 (P=0.03). Finally, there were sufficient evidence to confirm its value in the status of vascular invasion (P=0.01) and human papillomavirus (HPV) infection (P=0.02) in cervical cancer. More importantly, Kaplan-Meier analysis showed that cervical cancer patients with high miR-224 expression tend to have shorter overall survival. In multivariate analysis stratified for known prognostic variables, miR-224 was identified as an independent prognostic marker.
CONCLUSION
Our data indicated that miR-224 upregulation was associated with aggressive progression and poor prognosis in cervical cancer. MiR-224 was identified for the first time as an independent marker for predicting the clinical outcome of cervical cancer patients.
VIRTUAL SLIDES
The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/2170449349527493.
目的
越来越多的证据表明，微小 RNA-224（miR-224）在各种类型的人类癌症中的表达差异可能在肿瘤生物学中发挥关键作用。之前的微阵列检测还表明，miR-224 是宫颈癌组织中相对邻近正常组织显著上调的 miRNA 之一。然而，miR-224 在人类宫颈癌中的功能知之甚少。本研究旨在探讨 miR-224 表达在宫颈癌中的临床意义。
方法
通过实时定量 RT-PCR 检测 126 对新鲜人宫颈癌及相应邻近正常组织中 miR-224 的表达。
结果
与相应的邻近正常组织相比，宫颈癌组织中 miR-224 的表达显著上调（P<0.001）。FIGO 晚期宫颈癌患者的癌组织中 miR-224 的表达也明显高于 FIGO 早期患者（P=0.02）。此外，淋巴结转移阳性患者的 miR-224 表达水平明显高于淋巴结转移阴性患者（P=0.008）。此外，我们发现分化程度较低的肿瘤表达较高的 miR-224（P=0.03）。最后，有充分的证据证实其在宫颈癌血管侵犯（P=0.01）和人乳头瘤病毒（HPV）感染（P=0.02）状态下的价值。更重要的是，Kaplan-Meier 分析表明，miR-224 高表达的宫颈癌患者总生存时间较短。在分层考虑已知预后因素的多变量分析中，miR-224 被确定为独立的预后标志物。
结论
我们的数据表明，miR-224 的上调与宫颈癌的侵袭性进展和不良预后相关。miR-224 首次被确定为预测宫颈癌患者临床结局的独立标志物。
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