[来自动物粪便样本的嗜热弯曲杆菌及其在神经节苷脂（GM1）-酶联免疫吸附测定、Vero细胞试验和盐聚集试验中的行为]

[Thermophilic Campylobacter from fecal samples of animals and their behavior in the ganglioside (GM1)-ELISA, Vero cell test and salt aggregation test].

作者信息

Rakotozandrindraini R, Hamann H P, Zschöck M, Schliesser T

机构信息

Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität Giessen.

出版信息

Zentralbl Veterinarmed B. 1990 Mar;37(2):142-7.

PMID:2363323

Abstract

From 397 fecal specimens from apparently healthy and from diarrheic pigs, dogs, cats and cattle 59 strains (= 15%) of thermophilic Campylobacter (C.) spp. were isolated by culture. 39 strains were identified as C. coli and 18 as C. jejuni whereas 2 isolates could not be classified. None of the strains was found to be positive for cytotoxic enterotoxin in the GM1-ELISA. In the Vero-cell test 5 isolates showed a cytotoxic effect. The salt aggregation test (SAT) for indicating cell surface hydrophobicity was positive with 24 strains (5 C. jejuni, 19 C. coli). A correlation of isolation results with clinical manifestation could not be observed.

摘要

从397份来自看似健康及腹泻猪、狗、猫和牛的粪便样本中，通过培养分离出59株嗜热弯曲菌属菌株（占15%）。39株被鉴定为大肠弯曲菌，18株为空肠弯曲菌，另外2株无法分类。在GM1-ELISA检测中，未发现任何菌株的细胞毒性肠毒素呈阳性。在Vero细胞试验中，5株菌株显示出细胞毒性作用。用于指示细胞表面疏水性的盐聚集试验（SAT）中，24株菌株（5株空肠弯曲菌，19株大肠弯曲菌）呈阳性。未观察到分离结果与临床表现之间的相关性。