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柠檬苦素对HepG2细胞系中Wnt信号分子的影响。

Influence of limonin on Wnt signalling molecule in HepG2 cell lines.

作者信息

Langeswaran K, Gowthamkumar S, Vijayaprakash S, Revathy R, Balasubramanian M P

机构信息

Department of Industrial Biotechnology, Bharath University, Tambaram, India.

出版信息

J Nat Sci Biol Med. 2013 Jan;4(1):126-33. doi: 10.4103/0976-9668.107276.

DOI:10.4103/0976-9668.107276
PMID:23633848
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3633262/
Abstract

OBJECTIVE

The role of limonin as potent anti carcinogenic, apoptosis and chemotherapeutic agents has been supported by limited studies.

MATERIALS AND METHODS

In this study, limonin is identified as a potent anti proliferative agent against human hepatoma HepG2 cells based on the cell viability study, LDH leakage assay. Induction of apoptosis in HepG2 cells by limonin was evidenced by western blot analysis of Bax, Cyclin D1, Caspase 3 and Caspase9.

RESULTS

Since Wnt signalling is involved in the initiation and sustaining of hepatocellular carcinoma we studied differential expression of LRP5, LRP6 and DKK wnt players.

CONCLUSION

Limonin found to down regulate these players which forms a rationale for further investigation on effect on limonin in cancer therapy.

摘要

目的

有限的研究支持了柠檬苦素作为有效的抗癌、促凋亡和化疗药物的作用。

材料与方法

在本研究中，基于细胞活力研究、乳酸脱氢酶泄漏测定，柠檬苦素被确定为对人肝癌HepG2细胞有效的抗增殖剂。通过对Bax、细胞周期蛋白D1、半胱天冬酶3和半胱天冬酶9进行蛋白质印迹分析，证明柠檬苦素可诱导HepG2细胞凋亡。

结果

由于Wnt信号通路参与肝细胞癌的起始和维持，我们研究了低密度脂蛋白受体相关蛋白5（LRP5）、低密度脂蛋白受体相关蛋白6（LRP6）和Dickkopf相关蛋白1（DKK）等Wnt信号蛋白的差异表达。

结论

发现柠檬苦素可下调这些蛋白，这为进一步研究柠檬苦素在癌症治疗中的作用提供了理论依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd9c/3633262/367a1076f7b0/JNSBM-4-126-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd9c/3633262/97b8ad74e7f0/JNSBM-4-126-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd9c/3633262/50af73edd53f/JNSBM-4-126-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd9c/3633262/2cfead7c0f53/JNSBM-4-126-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd9c/3633262/304ace65914c/JNSBM-4-126-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd9c/3633262/fdc80b0c387a/JNSBM-4-126-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd9c/3633262/6710fc369ffd/JNSBM-4-126-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd9c/3633262/44ea421638e8/JNSBM-4-126-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd9c/3633262/4039df7b38d5/JNSBM-4-126-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd9c/3633262/29a74600949d/JNSBM-4-126-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd9c/3633262/299badbc6904/JNSBM-4-126-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd9c/3633262/e46be272778f/JNSBM-4-126-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd9c/3633262/367a1076f7b0/JNSBM-4-126-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd9c/3633262/97b8ad74e7f0/JNSBM-4-126-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd9c/3633262/50af73edd53f/JNSBM-4-126-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd9c/3633262/2cfead7c0f53/JNSBM-4-126-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd9c/3633262/304ace65914c/JNSBM-4-126-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd9c/3633262/fdc80b0c387a/JNSBM-4-126-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd9c/3633262/6710fc369ffd/JNSBM-4-126-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd9c/3633262/44ea421638e8/JNSBM-4-126-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd9c/3633262/4039df7b38d5/JNSBM-4-126-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd9c/3633262/29a74600949d/JNSBM-4-126-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd9c/3633262/299badbc6904/JNSBM-4-126-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd9c/3633262/e46be272778f/JNSBM-4-126-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd9c/3633262/367a1076f7b0/JNSBM-4-126-g012.jpg

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