双孔通道 1 的 N 端结构域通过 NAADP 调节其运输和激活。

The N-terminal region of two-pore channel 1 regulates trafficking and activation by NAADP.

机构信息

Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK.

出版信息

Biochem J. 2013 Jul 1;453(1):147-51. doi: 10.1042/BJ20130474.

DOI:10.1042/BJ20130474

Abstract

TPCs (two-pore channels) are NAADP (nicotinic acid-adenine dinucleotide phosphate)-sensitive Ca2+-permeable ion channels expressed on acidic organelles. In the present study we show that deletion of the N-terminal region redirects TPC1 to the ER (endoplasmic reticulum). The introduction of fluorophores at the N-terminus of TPC1 does not affect its subcellular location, but does reversibly abolish NAADP sensitivity. Our results reveal a dual role for the N-terminus in localization and function of TPC1.

摘要

TPCs（双孔通道）是一种在酸性细胞器上表达的 NAADP（烟酰胺腺嘌呤二核苷酸磷酸）敏感的 Ca2+通透性离子通道。在本研究中，我们发现 TPC1 的 N 端缺失会使其重新定向到内质网（ER）。在 TPC1 的 N 端引入荧光团不会影响其亚细胞定位，但可可逆地消除 NAADP 敏感性。我们的结果揭示了 N 端在 TPC1 定位和功能中的双重作用。