过氧化物酶体氧化酶的荧光测定法。
Fluorometric assay of peroxisomal oxidases.
作者信息
Vamecq J
机构信息
Laboratoire de Chimie Physiologique, International Institute of Cellular and Molecular Pathology, Brussels, Belgium.
出版信息
Anal Biochem. 1990 May 1;186(2):340-9. doi: 10.1016/0003-2697(90)90092-n.
The present paper deals with the adaptation of the fluorometric measurement of H2O2 originally described by Guilbault et al. (1967, Anal. Chem. 39, 271) for the assay of the peroxisomal oxidation of D-amino acids, L-alpha-hydroxyacids, uric acid, and acyl-CoA esters. The present work essentially covers three facets: (i) the general kinetics of the assay of peroxisomal oxidases and the influence of each component of the assay medium on these kinetics; (ii) the measurement of peroxisomal oxidase activities in subcellular fractions and tissues from human and untreated and clofibrate-treated rodents; and (iii) the comparison between the oxidase activities measured by the fluorometric and spectrophotometric methods.
本文论述了对Guilbault等人（1967年，《分析化学》39卷，271页）最初描述的用于检测D-氨基酸、L-α-羟基酸、尿酸和酰基辅酶A酯的过氧化物酶体氧化的H2O2荧光测量法进行的改进。目前的工作主要涵盖三个方面：（i）过氧化物酶体氧化酶检测的一般动力学以及检测介质各成分对这些动力学的影响；（ii）人以及未处理和用氯贝丁酯处理的啮齿动物的亚细胞组分和组织中过氧化物酶体氧化酶活性的测量；（iii）荧光法和分光光度法测量的氧化酶活性之间的比较。
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