Department of Anatomical Pathology, PathWest, QEII Medical Centre, Nedlands, Western Australia, Australia.
Pathology. 2013 Jun;45(4):402-7. doi: 10.1097/PAT.0b013e328360c064.
The aim of this study was to document the pattern of immunohistochemical staining seen with p16 (INK4a) in actinic keratosis, Bowen's disease and seborrhoeic keratosis.
We gathered 20 examples each of actinic keratosis, Bowen's disease and seborrheic keratosis. The cases were stained for p16 using standard immunohistochemical techniques, and the staining patterns were categorised into one of five different patterns.
All cases of Bowen's disease as defined in our practice showed strong positive staining in all abnormal cells, and 95% of these cases showed a distinctive pattern of sparing in a layer of palisaded basal cells. None of the actinic keratoses or seborrheic keratoses, as defined by our morphological criteria, showed this distinctive pattern.
Bowen's disease, as we define the term, shows a distinctive, repeatable pattern of staining with p16, characterised by moderate to strong staining of all abnormal cells with sparing of a layer of basal cells. This pattern is not seen in actinic keratoses or in seborrheic keratoses. Thus immunohistochemistry for p16 is a useful adjunctive test in the differential diagnosis of these lesions.
本研究旨在记录 p16（INK4a）在光化性角化病、鲍文病和脂溢性角化病中的免疫组织化学染色模式。
我们收集了 20 例光化性角化病、鲍文病和脂溢性角化病。使用标准免疫组织化学技术对 p16 进行染色，并将染色模式分为五种不同模式之一。
在我们的实践中定义的所有鲍文病病例均在所有异常细胞中显示出强阳性染色，其中 95%的病例显示出一种独特的基底层细胞分层保留模式。我们形态学标准定义的光化性角化病或脂溢性角化病均未显示出这种独特的模式。
如我们所定义的术语，鲍文病显示出一种独特的、可重复的 p16 染色模式，其特征是所有异常细胞均有中度至强染色，而基底层细胞有一层保留。这种模式在光化性角化病或脂溢性角化病中均未出现。因此，p16 的免疫组织化学检查是这些病变鉴别诊断的有用辅助检查。
