Department of Bio-Interaction Sciences, Federal University of Bahia, Av. Reitor Miguel Calmon, s/n, Vale do Canela, Salvador, Bahia, 40110-902, Brazil,
Bioprocess Biosyst Eng. 2013 Dec;36(12):1861-9. doi: 10.1007/s00449-013-0960-0. Epub 2013 May 1.
The activity of anaerobic sulfate reduction was studied using sulfate-reducing bacteria isolated from the water produced from a Brazilian oil reservoir. The effects of the initial sulfate concentration on the anaerobic sulfate reduction and sulfide generation kinetics were investigated. The redox potential, the biomass solution content, and the sulfate and the sulfide solution content were measured. The results indicate that the sulfate conversion and the sulfide generation are both first-order processes for the initial sulfate concentration of 823, 1,282, and 1,790 mg/L. The results for the kinetic constants for the sulfate conversion indicate an inhibition with the enhancement of the initial sulfate solution content. The kinetic constants for the sulfide generation indicate that this reaction is almost independent of the initial sulfate solution content due to the presence of at least two in-series processes that are faster than the microbial conversion of the sulfate. The kinetic test using the water from an onshore oil field, with an initial sulfide content of 228 mg/L and sulfate content of 947 mg/L, shows a sulfate conversion of 50 % in 528 h. The kinetic modeling for the net content of sulfate and sulfide indicates that the sulfate conversion is slower for this water than for the deionized water tests; however, the sulfide formation has almost the same conversion velocity. The reactions are first order in both cases.
采用从巴西油田产出水中分离出的硫酸盐还原菌，研究了厌氧硫酸盐还原活性。考察了初始硫酸盐浓度对厌氧硫酸盐还原和硫化物生成动力学的影响。测定了氧化还原电位、生物量溶液含量以及硫酸盐和硫化物溶液含量。结果表明，当初始硫酸盐浓度分别为 823、1282 和 1790mg/L 时，硫酸盐转化和硫化物生成均为一级过程。硫酸盐转化的动力学常数表明，随着初始硫酸盐溶液含量的增加，存在抑制作用。硫化物生成的动力学常数表明，由于至少存在两个串联过程，其比硫酸盐的微生物转化更快，因此该反应几乎与初始硫酸盐溶液含量无关。利用初始硫化物含量为 228mg/L 和硫酸盐含量为 947mg/L 的陆上油田水进行的动力学试验表明，528h 内硫酸盐转化率为 50%。硫酸盐和硫化物净含量的动力学模型表明，对于该水样，硫酸盐的转化率比去离子水试验慢；但是，硫化物的形成具有几乎相同的转化速度。两种情况下的反应均为一级反应。
