将全藻荧光素酶显微注射到去卵壳的完整斑马鱼胚胎中。
Microinjecting holo-aequorin into dechorionated and intact zebrafish embryos.
作者信息
Webb Sarah E, Miller Andrew L
机构信息
Division of Life Science and State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, Hong Kong, People's Republic of China.
出版信息
Cold Spring Harb Protoc. 2013 May 1;2013(5):447-55. doi: 10.1101/pdb.prot072967.
The injection of holo-aequorin into embryos at the one-cell stage, along with the use of a simple photomultiplier tube or luminescence imaging system, allows transient localized elevations of free cytosolic Ca(2+) to be recorded and observed during the first 24 h of zebrafish development. The technique for loading dechorionated or intact one-cell stage zebrafish embryos with holo-aequorin is described here.
在单细胞阶段将全藻钙蛋白注射到胚胎中，同时使用简单的光电倍增管或发光成像系统，能够记录和观察斑马鱼发育最初24小时内游离胞质Ca(2+)的瞬时局部升高。本文描述了用全藻钙蛋白加载去绒毛膜或完整的单细胞阶段斑马鱼胚胎的技术。
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