Doulakas J
J Pharm Sci. 1975 Feb;64(2):307-10. doi: 10.1002/jps.2600640225.
A colorimetric method was developed for the rapid analysis of bacitracin in ophthalmic ointments. The method involves the oxidation of alpha-aminocarboxylic acid with sodium hypobromite in an alkaline medium and condensation of the resulting aldehyde with phloroglucinol in concentrated hydrochloric acid to yield a pink color which gives an absorbance maximum at 505 nm. The relationship between absorbance and the quantity of bacitracin reacted obeyed Beer's law over the 15-100-mug/ml concentration range studied, and the produced color was stable for several hours. Furthermore, the method, which can be applied directly to the aqueous dissolution sample, gave results comparable to the official microbiological analytical procedure. The standard deviation is equal to plus or minus 1.81%.
开发了一种比色法用于快速分析眼药膏中的杆菌肽。该方法包括在碱性介质中用次溴酸钠氧化α-氨基羧酸，并使生成的醛与浓盐酸中的间苯三酚缩合，产生粉红色，在505nm处有最大吸光度。在所研究的15 - 100μg/ml浓度范围内，吸光度与反应的杆菌肽量之间的关系符合比尔定律，并且产生的颜色在数小时内稳定。此外，该方法可直接应用于水溶解样品，所得结果与官方微生物分析程序相当。标准偏差等于±1.81%。
