West China School of Public Health, Sichuan University, P. R. China.
J Environ Sci Health B. 2013;48(8):637-45. doi: 10.1080/03601234.2013.777308.
The present study was designed to investigate the immunotoxicity of atrazine (ATZ) in male Balb/c mice. ATZ (175, 87.5, and 43.75 mg/kg bw/day) was administered by gavage method for 28 days. The following indexes were determined in various groups of mice: body and organ weight; antibody aggregation of serum hemolysin; proliferative response of splenocytes to ConA; delayed-type hypersensitivity (DTH); natural killer cell activity; clearance of neutral red and nitric oxide (NO) release from peritoneal macrophages; apostosis and necrosis of splenocytes and thymocytes; cytokine production; and serum lysozyme. Results showed that cell-mediated, humoral immunity, and non-specific immune function in the high-dose ATZ group were suppressed; NO release and interferon-γ(IFN-γ)/interleukin-4 (IL-4) were also significantly decreased in the high-dose group. In the medium-dose group, the proliferation response and IFN-γ production was significantly decreased. In the low-dose group, the proliferation response was significantly decreased. Serum lysozyme was decreased in the ATZ-treated groups. The percentage of early apoptosis in thymocytes was increased significantly in high- and medium-dose ATZ groups. In conclusion, ATZ elicited an inhibitory effect on cell-mediated immunity, humoral immunity, and non-specific immune function of mice.
本研究旨在探讨莠去津（ATZ）对雄性 Balb/c 小鼠的免疫毒性。采用灌胃法给小鼠染毒 28 天，染毒剂量分别为 175、87.5 和 43.75 mg/kg bw/day。测定各组小鼠的下列指标：体重和器官重量；血清溶血素抗体聚集；刀豆蛋白 A 刺激的脾细胞增殖反应；迟发型超敏反应（DTH）；自然杀伤细胞活性；中性红清除率和腹腔巨噬细胞一氧化氮（NO）释放；脾细胞和胸腺细胞凋亡和坏死；细胞因子产生；血清溶菌酶。结果表明，高剂量 ATZ 组细胞介导的、体液免疫和非特异性免疫功能受到抑制；高剂量组 NO 释放和干扰素-γ（IFN-γ）/白细胞介素-4（IL-4）也显著降低。中剂量组增殖反应和 IFN-γ 产生显著降低。低剂量组增殖反应显著降低。ATZ 处理组血清溶菌酶降低。高、中剂量 ATZ 组胸腺细胞早期凋亡率显著增加。结论：莠去津对小鼠的细胞免疫、体液免疫和非特异性免疫功能均有抑制作用。
