College of Agriculture, Ludong University, Yantai, PR China.
J Invertebr Pathol. 2013 Jul;113(3):237-9. doi: 10.1016/j.jip.2013.04.009. Epub 2013 Apr 29.
Infectious myonecrosis virus (IMNV) is a recently observed shrimp virus, which threats the cultured Litopenaeus vannamei and can cause huge economic loss in shrimp farming industry. The specific aim of this study was to develop a new sensitive real-time PCR method for the specific detection of shrimp IMNV. A real-time PCR assay with a pair of primers to specifically amplify a 101bp IMNV cDNA fragment and a corresponding TaqMan probe was developed, which shown to be specific for IMNV without cross reaction with DNA samples prepared from four other shrimp viruses including white spot syndrome virus (WSSV), hepatopancreatic parvovirus (HPV), monodon baculovirus (MBV), and infectious hypodermal and haematopoietic virus (IHHNV). The method could detect as low as one single copy of IMNV plasmid cDNA.
传染性肌肉坏死病毒（IMNV）是一种最近观察到的虾病毒，它威胁着养殖的凡纳滨对虾，并可能给虾养殖业造成巨大的经济损失。本研究的目的是开发一种新的敏感实时 PCR 方法，用于特异性检测虾传染性肌肉坏死病毒。开发了一种实时 PCR 检测方法，使用一对引物特异性扩增 101bp 的 IMNV cDNA 片段和相应的 TaqMan 探针，该方法对 IMNV 具有特异性，与从其他四种虾病毒（包括白斑综合征病毒（WSSV）、肝胰腺细小病毒（HPV）、对虾杆状病毒（MBV）和传染性皮下及造血组织坏死病毒（IHHNV））制备的 DNA 样本无交叉反应。该方法可检测到低至一个拷贝的 IMNV 质粒 cDNA。
