磁珠组装的旋磁作为一种蛋白质检测的信号转导方法。
Bead assembly magnetorotation as a signal transduction method for protein detection.
机构信息
Department of Biomedical Engineering, University of Michigan, 2200 Bonisteel Blvd., Ann Arbor, MI 48109, USA.
出版信息
Biosens Bioelectron. 2013 Oct 15;48:26-32. doi: 10.1016/j.bios.2013.03.073. Epub 2013 Apr 6.
Abstract
This paper demonstrates a proof-of-principle for a new signal transduction method for protein detection called Bead Assembly Magnetorotation (BAM). BAM is based on using the target protein to mediate the formation of aptamer-coated 1 μm magnetic beads into a bead assembly, formed at the bottom of a 1 μL hanging droplet. The size, shape and fractal dimension of this bead assembly all depend on the protein concentration. The protein concentration can be measured in two ways: by magnetorotation, in which the rotational period of the assembly correlates with the protein concentration, or by fractal analysis. Additionally, a microscope-free magnetorotation detection method is introduced, based on a simple laser apparatus built from standard laboratory components. In this paper, we chose to focus on the protein thrombin, a popular choice for proof-of-principle work in this field.
摘要
本文展示了一种名为磁珠组装旋转（BAM）的新型蛋白质检测信号转导方法的原理验证。BAM 基于利用目标蛋白来介导适配体涂层的 1μm 磁性珠形成珠组装体，该组装体在 1μL 悬挂液滴的底部形成。该珠组装体的大小、形状和分形维数都取决于蛋白质浓度。可以通过两种方法测量蛋白质浓度：通过磁旋转，其中组装体的旋转周期与蛋白质浓度相关联，或者通过分形分析。此外，还引入了一种无显微镜的磁旋转检测方法，该方法基于由标准实验室组件构建的简单激光设备。在本文中，我们选择关注蛋白质凝血酶，这是该领域原理验证工作的热门选择。
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