肉碱棕榈酰转移酶的抑制导致大鼠肝脏中3-羟基-3-甲基戊二酰辅酶A还原酶活性的诱导。

Inhibition of carnitine palmitoyltransferase leads to induction of 3-hydroxymethylglutaryl coenzyme A reductase activity in rat liver.

作者信息

Jansen H, Hoogerbrugge Van der Linden N, Hülsmann W C

机构信息

Department of Biochemistry, Medical Faculty, Erasmus University Rotterdam, The Netherlands.

出版信息

Biochim Biophys Acta. 1990 Jun 14;1044(3):390-3. doi: 10.1016/0005-2760(90)90267-2.

DOI:10.1016/0005-2760(90)90267-2

PMID:2364104

Abstract

The relation between carnitine palmitoyltransferase (CPT) activity and 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase activity was investigated. Rats were treated with aminocarnitine or 1-carnitine overnight. In rats, in which CPT activity was inhibited by aminocarnitine, plasma and hepatic triacylglycerol contents were increased 5- to 6-fold. The plasma cholesterol concentration was unchanged, while the hepatic cholesterol content was lowered (-16%). Hepatic cholesterol synthesis, determined by following the incorporation of 14C-acetate and 3H2O into digitonin-precipitable sterols, in liver slices was increased 5- to 7-fold. HMG-CoA reductase activity in liver microsomes was increased to the same extent.

摘要

研究了肉碱棕榈酰转移酶（CPT）活性与3-羟基-3-甲基戊二酰辅酶A（HMG-CoA）还原酶活性之间的关系。用氨基肉碱或左旋肉碱对大鼠进行过夜处理。在氨基肉碱抑制CPT活性的大鼠中，血浆和肝脏三酰甘油含量增加了5至6倍。血浆胆固醇浓度未变，而肝脏胆固醇含量降低了（-16%）。通过追踪14C-乙酸盐和3H2O掺入肝切片中洋地黄皂苷可沉淀甾醇的情况来测定的肝脏胆固醇合成增加了5至7倍。肝微粒体中的HMG-CoA还原酶活性也增加到相同程度。