Hamid Muzamil M Abdel, Mohammed Sara B, El Hassan Ibrahim M
Department of Molecular Biology, Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan.
N Am J Med Sci. 2013 Feb;5(2):95-101. doi: 10.4103/1947-2714.107524.
Characterization of Plasmodium falciparum diversity is commonly achieved by amplification of the polymorphic regions of the merozoite surface proteins 1 (MSP1) and 2 (MSP2) genes.
The present study aimed to determine the allelic variants distribution of MSP1 and MSP2 and multiplicity of infection in P. falciparum field isolates from Kosti, central Sudan, an area characterized by seasonal malaria transmission.
Total 121 samples (N = 121) were collected during a cross-sectional survey between March and April 2003. DNA was extracted and MSP1 and MSP2 polymorphic loci were genotyped.
The total number of alleles identified in MSP1 block 2 was 11, while 16 alleles were observed in MSP2 block 3. In MSP1, RO33 was found to be the predominant allelic type, carried alone or in combination with MAD20 and K1 types, whereas FC27 family was the most prevalent in MSP2. Sixty two percent of isolates had multiple genotypes and the overall mean multiplicity of infection was 1.93 (CI 95% 1.66-2.20). Age correlated with parasite density (P = 0.017). In addition, a positive correlation was observed between parasite densities and the number of alleles (P = 0.022).
Genetic diversity in P. falciparum field isolates in central Sudan was high and consisted of multiple clones.
恶性疟原虫多样性的特征通常通过扩增裂殖子表面蛋白1（MSP1）和2（MSP2）基因的多态性区域来实现。
本研究旨在确定苏丹中部科斯蒂地区恶性疟原虫野外分离株中MSP1和MSP2的等位基因变体分布以及感染复数，该地区以季节性疟疾传播为特征。
在2003年3月至4月的横断面调查中总共收集了121份样本（N = 121）。提取DNA并对MSP1和MSP2多态性位点进行基因分型。
在MSP1第2区鉴定出的等位基因总数为11个，而在MSP2第3区观察到16个等位基因。在MSP1中，RO33被发现是主要的等位基因类型，单独携带或与MAD20和K1类型组合携带，而FC27家族在MSP2中最为普遍。62%的分离株具有多种基因型，总体平均感染复数为1.93（95%置信区间1.66 - 2.20）。年龄与寄生虫密度相关（P = 0.017）。此外，在寄生虫密度和等位基因数量之间观察到正相关（P = 0.022）。
苏丹中部恶性疟原虫野外分离株的遗传多样性很高，由多个克隆组成。
