Kravchenko M N, Ausheva L Kh, Bendikov E A
Biull Eksp Biol Med. 1990 Mar;109(3):252-4.
Guinea pigs received skin application of petroleum-derived mineral oil distillate, containing about 10% of polycyclic aromatic hydrocarbons with the 4 hours exposure time everyday during 20 days. 100% distillate preparation and it's 50%, 3% or 0.5% solutions in furfurol and ethanol are used. It resulted in the distillate-dose-dependent cytochrome P-450 induction (1.38-2.23-fold) in liver of the all exposed groups of guinea pigs and in 20-60% decrease in microsomal and cytosol glutathione transferase activities in groups which received 50% and 3% mineral oil distillate solutions. Ratio values of cytochrome P-450 content level to glutathiontransferase activity level depended linearly on the distillate doses, and it increased 2.7-4.4-fold with the distillate concentration increasing in the preparation from 0.5% to 50%. Conclusion was made that with increasing distillate doses the process of polycyclic aromatic hydrocarbon activation with the genotoxic metabolite formation predominated over the process of those metabolite detoxication.
豚鼠每天皮肤涂抹含有约10%多环芳烃的石油衍生矿物油馏出物，暴露时间为4小时，持续20天。使用100%馏出物制剂及其在糠醛和乙醇中的50%、3%或0.5%溶液。结果显示，所有暴露组豚鼠肝脏中细胞色素P-450的诱导呈馏出物剂量依赖性（1.38 - 2.23倍），而接受50%和3%矿物油馏出物溶液的组中，微粒体和胞质溶胶谷胱甘肽转移酶活性降低了20 - 60%。细胞色素P-450含量水平与谷胱甘肽转移酶活性水平的比值与馏出物剂量呈线性关系，且随着制剂中馏出物浓度从0.5%增加到50%，该比值增加了2.7 - 4.4倍。得出的结论是，随着馏出物剂量的增加，多环芳烃激活并形成遗传毒性代谢物的过程比这些代谢物的解毒过程更为突出。
