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[杂交瘤细胞培养物中脂褐素的检测]

[The detection of lipofuscin in a culture of hybridoma cells].

作者信息

Tatariunas A B, Popov V I, Mantsygin Iu A, Karnaukhov V N

出版信息

Biull Eksp Biol Med. 1990 Mar;109(3):259-62.

PMID:2364152
Abstract

Lipofuscin granules (LG) are found in the cultured hybridoma cells producing monoclonal antibodies to phage. LG have been studied using light and electron microscopy. Luminescent spectra of LG clusters in hybridoma cells are presented. The increase of own luminescence intensity of LG in the course of excitation by ultraviolet (365/nm) is shown. The advantages of hybridoma cells culture for investigation of LG on the cell level are discussed.

摘要

脂褐素颗粒(LG)存在于产生噬菌体单克隆抗体的培养杂交瘤细胞中。已使用光学显微镜和电子显微镜对LG进行了研究。给出了杂交瘤细胞中LG簇的发光光谱。显示了在紫外线(365纳米)激发过程中LG自身发光强度的增加。讨论了利用杂交瘤细胞培养在细胞水平上研究LG的优势。

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