Bioforsk, Norwegian Institute for Agricultural and Environmental Research, Frederik A. Dahls vei 20, 1432 ÅS, Norway.
Anim Reprod Sci. 2013 Jun;139(1-4):155-61. doi: 10.1016/j.anireprosci.2013.03.017. Epub 2013 Apr 11.
The present study on wild caught Atlantic cod Gadus morhua provides a detailed description of the spermatozoa ultrastructure. The spermatozoa of G. morhua are composed of a head (nucleus) with no acrosome, a midpiece and a single flagellum. The total length is 67.69±6.33μm with a lanceolate sperm head that is 2.34±0.26μm long and 1.32±0.26μm wide. The nucleus presents homogeneous condensation of chromatin with few lacunae of decondensed chromatin. The nuclear fossa penetrates over three third of the nuclear axis and is tubiform. The axoneme is composed of a typical 9+2 microtubular doublet structure and is enclosed by the plasma membrane. Central microtubules are absent from the proximal portion of the anterior end of nuclear notch and a distal centriole complex is perpendicular to the axoneme. A group of mitochondria and vesicles is present in the midpiece. The ultrastructure of the Gadidae G. morhua spermatozoon differs from the structures described earlier for other Gadiformes. The unique features may be synapomorphic for marine Gadidae, and may contribute to the study of phylogenetic relationships in teleost fishes, and be a reference for future studies on spermatozoon quality and morphology in fish.
本研究对野生捕捞大西洋鳕鱼（Gadus morhua）的精子超微结构进行了详细描述。G. morhua 的精子由头部（核）、中段和一条鞭毛组成，没有顶体。全长 67.69±6.33μm，头部长而呈 lanceolate，长 2.34±0.26μm，宽 1.32±0.26μm。核呈现出染色质均匀凝聚，有少量去凝聚染色质的腔隙。核窝穿透核轴的三分之二以上，呈管状。轴丝由典型的 9+2 微管二联体结构组成，被质膜包裹。近端核切迹前端没有中央微管，远端的中心粒复合体垂直于轴丝。中段有一组线粒体和囊泡。Gadidae G. morhua 精子的超微结构与先前描述的其他 Gadiformes 的结构不同。这些独特的特征可能是海洋 Gadidae 的共衍征，有助于研究硬骨鱼类的系统发育关系，并为鱼类精子质量和形态的未来研究提供参考。
