Department of Surgical, Medical, Dental and Morphological Sciences with interest in Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, Modena, Italy.
Placenta. 2013 Jul;34(7):526-35. doi: 10.1016/j.placenta.2013.03.017. Epub 2013 Apr 30.
Human term placenta (HTP) has attracted increasing attention as an alternative source of stem cells for regenerative medicine since the amniochorionic membrane harbors stem cells populations that are easily accessible, abundantly available without ethical objections. In the chorionic side of HTP we found a progenitor perivascular "niche" in which rare cells co-express Oct-4 and c-Kit. We investigated the stem cell characteristics and differentiation potential of a chorionic derived population enriched in c-Kit(+) cells and compared this to the unenriched population.
Cells, isolated from the chorion of HTP, were expanded and enriched in c-Kit(+) cells (Chorionic Stem Cells-CSC). Histological staining, immunofluorescence, Western blot and flow cytometry were used to verify the stem cells characteristics of the populations and to compare the differentiation capability towards mesodermal and neural lineages in vitro.
The expression of the pluripotent marker Oct-4 was greater in the CSCs compared to the unselected cells (Chorionic Cell-CC) but both Oct-4 and c-Kit expression decreased during passages. After differentiation, CSC displayed stronger chondrogenic and osteogenic potential and a greater adipogenic forming capacity compared to unselected ones. CSC differentiated better into immature oligodendrocytes while CC showed a neuronal progenitor differentiation potential. Moreover, both populations were able to differentiate in hepatogenic lineage.
CSC display improved Oct-4 expression and a high differentiation potential into mesodermal lineages and oligodendrocytes.
人类胎盘（HTP）作为再生医学中干细胞的替代来源，因其羊膜绒毛膜中含有易于获取的、丰富的且不存在伦理问题的干细胞群体而受到越来越多的关注。我们在 HTP 的绒毛膜侧发现了一个祖细胞血管周“龛”，其中稀有细胞共表达 Oct-4 和 c-Kit。我们研究了富含 c-Kit(+)细胞的绒毛膜来源群体的干细胞特性和分化潜能，并将其与未富集群体进行了比较。
从 HTP 的绒毛膜中分离出的细胞进行了扩增，并富集 c-Kit(+)细胞（绒毛膜干细胞-CSC）。组织学染色、免疫荧光、Western blot 和流式细胞术用于验证群体的干细胞特性，并比较体外向中胚层和神经谱系的分化能力。
多能标志物 Oct-4 在 CSC 中的表达高于未选择的细胞（绒毛膜细胞-CC），但在传代过程中 Oct-4 和 c-Kit 的表达均降低。分化后，CSC 表现出更强的软骨和成骨潜力，以及更大的成脂形成能力，与未选择的细胞相比。CSC 向未成熟少突胶质细胞分化的能力更强，而 CC 显示出神经元祖细胞分化的潜力。此外，这两种群体都能够向肝谱系分化。
CSC 显示出更高的 Oct-4 表达和向中胚层谱系和少突胶质细胞分化的高潜能。
