Ma Zhiyuan, Xie Yumei, Wang Yu, Ma Li, He Yu, Zhang Ying, Lian Jianqi, Guo Yonghong, Jia Zhansheng
PLA Center for Diagnosis and Treatment of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2013 May;29(5):515-8, 521.
To detect cell frequency and surface markers of peripheral blood CD4⁺;CXCR5⁺;follicular helper T cells (Tfh cells) and analyze the correlation between CD4⁺;CXCR5⁺;Tfh cells and hyperglobulinemia of patients with chronic hepatitis B.
We collected blood samples of 20 HBV infected patients with hyperglobulinemia, 10 chronic HBV infected patients and 10 health volunteers and isolated plasma and peripheral blood mononuclear cells (PBMCs). The percentage of CD4⁺;CXCR5⁺;Tfh cells and the expressions of PD-1, ICOS and CD40L on CD4⁺;CXCR5⁺;Tfh cells were detected by flow cytometry. The levels of CXCL13, IFN-γ and IL-21 in plasma were measured by ELISA.
Compared with the percentage of CD4⁺;CXCR5⁺;Tfh cells in chronic HBV infected patients (11.9 ± 3.9) and health controls (6.8 ± 3.9), it was higher in HBV infected patients with hyperglobulinemia (22.6 ± 4.7, P<0.05). And in the hyperglobulinemia patients, the expressions of PD-1 and CD40L on CD4⁺;CXCR5⁺;Tfh cells and the levels of CXCL13 and IL-21 in plasma increased, whereas the level of IFN-γ significantly declined (P<0.05).
The results suggest that CD4⁺;CXCR5⁺;Tfh cells may participate in the pathogenesis of hyperglobulinemia during HBV infection.
检测外周血CD4⁺;CXCR5⁺;滤泡辅助性T细胞（Tfh细胞）的细胞频率和表面标志物，并分析慢性乙型肝炎患者CD4⁺;CXCR5⁺;Tfh细胞与高球蛋白血症之间的相关性。
收集20例高球蛋白血症的HBV感染患者、10例慢性HBV感染患者和10名健康志愿者的血样，分离血浆和外周血单个核细胞（PBMC）。采用流式细胞术检测CD4⁺;CXCR5⁺;Tfh细胞的百分比以及CD4⁺;CXCR5⁺;Tfh细胞上PD-1、ICOS和CD40L的表达。采用ELISA法检测血浆中CXCL13、IFN-γ和IL-21的水平。
与慢性HBV感染患者（11.9±3.9）和健康对照者（6.8±3.9）中CD4⁺;CXCR5⁺;Tfh细胞的百分比相比，高球蛋白血症的HBV感染患者中该细胞百分比更高（22.6±4.7，P<0.05）。并且在高球蛋白血症患者中，CD4⁺;CXCR5⁺;Tfh细胞上PD-1和CD40L的表达以及血浆中CXCL13和IL-21的水平升高，而IFN-γ水平显著下降（P<0.05）。
结果表明CD4⁺;CXCR5⁺;Tfh细胞可能参与HBV感染期间高球蛋白血症的发病机制。
