College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310014, PR China.
Int J Biol Macromol. 2013 Aug;59:295-300. doi: 10.1016/j.ijbiomac.2013.04.048. Epub 2013 May 2.
Four different molecular weight sulfated polysaccharides (UFP1, UFP2, UFP3 and UFP4) were extracted and separated from Ulva fasciata by hot water extraction and ultrafiltration. Their chemical and physical characteristics were determined and antioxidant activities were investigated on the basis of superoxide radical assay, hydroxyl radical assay, ABTS radical assay, and reducing power assay. The results showed that four polysaccharides exhibited antioxidant properties, and UFP2 and UFP3, which have lower content of sulfate showed higher antioxidant activities than UFP1 and UFP4, which have higher content of sulfate. Besides, the content of protein, uronic acid and molecular weights of polysaccharides also influence their antioxidant activities. The antioxidant activities of UFP were not a function of a single factor but a combination of several factors.
四种不同分子量的硫酸化多糖（UFP1、UFP2、UFP3 和 UFP4）通过热水提取和超滤从石莼中提取和分离。基于超氧自由基测定、羟基自由基测定、ABTS 自由基测定和还原力测定，确定了它们的化学和物理特性，并研究了抗氧化活性。结果表明，四种多糖均具有抗氧化活性，硫酸根含量较低的 UFP2 和 UFP3 比硫酸根含量较高的 UFP1 和 UFP4 具有更高的抗氧化活性。此外，多糖的蛋白质、糖醛酸含量和分子量也影响其抗氧化活性。UFP 的抗氧化活性不是单一因素的函数，而是几个因素的组合。
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