College of Light Industry and Food Sciences, South China University of Technology, Guangdong Province, Guangzhou, China.
Int J Biol Macromol. 2012 Jun 1;50(5):1183-7. doi: 10.1016/j.ijbiomac.2012.03.019. Epub 2012 Mar 30.
Five polysaccharides (IOP1b, IOP2a, IOP2c, IOP3a and IOP4) were isolated and purified from Inonotus obliquus by DEAE-Sepharose fast flow and SepharoseCL-6B column chromatography. Their chemical and physical characteristics were determined and antioxidant activities were investigated on the basis of hydroxyl radical assay, superoxide radical assay and ferric-reducing antioxidant power assay. The results showed that five polysaccharides exhibited antioxidant activities, and the higher content of uronic acid and proteinous substances, the stronger antioxidant activities of polysaccharides. Besides, molecular weights of polysaccharides also influence their antioxidant activities. IOP3a and IOP4 showed higher antioxidant properties than IOP1b, IOP2a and IOP2c.
从斜生厚孔菌中分离纯化得到 5 种多糖（IOP1b、IOP2a、IOP2c、IOP3a 和 IOP4）。通过 DEAE-Sepharose 快速流动和 SepharoseCL-6B 柱层析对其进行分离纯化。基于羟基自由基测定、超氧自由基测定和铁还原抗氧化能力测定，确定了它们的化学和物理特性，并研究了它们的抗氧化活性。结果表明，5 种多糖均具有抗氧化活性，且多糖中含有较高的糖醛酸和蛋白质含量，具有较强的抗氧化活性。此外，多糖的分子量也影响其抗氧化活性。IOP3a 和 IOP4 表现出比 IOP1b、IOP2a 和 IOP2c 更高的抗氧化性能。
