印度患者脑膜瘤的分子特征：22、17、14 和 10 号染色体杂合性缺失分析。

Molecular characteristics of meningiomas in a cohort of Indian patients: loss of heterozygosity analysis of chromosomes 22, 17, 14 and 10.

机构信息

Department of Pathology and Neurological Sciences, Christian Medical College, Vellore, Tamil Nadu, India.

出版信息

Neurol India. 2013 Mar-Apr;61(2):138-43. doi: 10.4103/0028-3886.111119.

DOI:10.4103/0028-3886.111119

PMID:23644312

Abstract

BACKGROUND

Though, loss of heterozygosity (LOH) at chromosome 22q is considered to be the most likely initiating event in the formation of meningiomas, LOH at other chromosomes (1, 3, 6, 9, 10, 11, 14.17, and 18) have been implicated in its progression. The aim of this study was to analyze microsatellite markers on a select set of chromosomes including, 22q, 10q, 14q, and 17p for LOH in patients with meningiomas.

MATERIALS AND METHODS

Tumor tissue and its corresponding blood sample were collected from 27 patients with meningioma. Four polymorphic microsatellite markers (D10S520, D17S1289, D14S555, and D22S417) were characterized for LOH analysis.

RESULTS

There were 14 World Health Organization (WHO) grade I, 12 WHO grade II and 1 WHO grade III meningiomas. LOH was seen most often at D22S417 with an equal distribution between the grades (33% of informative samples in each grade). Though, LOH at D14S555 was seen in 50% of informative WHO grade II tumors, compared to 11.1% of informative WHO grade I tumors it did not reach statistical significance. However, allelic imbalance (AI) at D14S555 was significantly associated with atypia (P = 0.05). LOH at D17S1289 was seen only in one tumor sample, and none of the informative samples displayed LOH at D10S520.

CONCLUSION

The frequency and equal distribution of LOH at chromosome 22 supports the hypothesis that it is an early event in the tumorigenesis of meningiomas. The association of AI at D14S555 in WHO grade II meningiomas needs to be investigated on a larger set of samples.

摘要

背景

虽然染色体 22q 的杂合性丢失（LOH）被认为是脑膜瘤形成的最可能的起始事件，但其他染色体（1、3、6、9、10、11、14.17 和 18）的 LOH 也与脑膜瘤的进展有关。本研究旨在分析包括 22q、10q、14q 和 17p 在内的一组选定染色体上的微卫星标记，以检测脑膜瘤患者的 LOH。

材料和方法

从 27 名脑膜瘤患者中采集肿瘤组织及其相应的血液样本。对 4 个多态性微卫星标记（D10S520、D17S1289、D14S555 和 D22S417）进行 LOH 分析。

结果

有 14 例世界卫生组织（WHO）I 级、12 例 II 级和 1 例 III 级脑膜瘤。LOH 最常见于 D22S417，各级别之间分布均匀（每个级别中有信息的样本中 33%出现 LOH）。然而，D14S555 的 LOH 在 50%的有信息的 II 级 WHO 肿瘤中可见，而在 11.1%的有信息的 I 级 WHO 肿瘤中未见，差异无统计学意义。然而，D14S555 上的等位基因不平衡（AI）与非典型性显著相关（P=0.05）。D17S1289 的 LOH 仅见于一个肿瘤样本，且无信息的样本均未见 D10S520 的 LOH。