Vieira C A, Toledo L F, Longcore J E, Longcore J R
Laboratório de Antígenos Bacterianos II, Departamento Microbiologia e Imunologia, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brazil.
Braz J Biol. 2013 Feb;73(1):195-9. doi: 10.1590/s1519-69842013000100021.
A fungal pathogen Batrachochytrium dendrobatidis (Bd), which can cause morbidity and death of anurans, has affected amphibian populations on a worldwide basis. Availability of pure cultures of Bd isolates is essential for experimental studies to understand the ecology of this pathogen. We evaluated the relationships of body length of Hylodes cf. ornatus and Lithobates catesbeianus tadpoles to depigmentation of mouthparts and determined if dekeratinization indicated an infection by Batrachochytrium dendrobatidis. A strong association existed for both species, one from South America (Brazil: São Paulo) and one from North America (USA: Maine). We believe it prudent not to kill adult amphibians if avoidable, thus obtaining tissue for isolating Bd from tadpoles is reasonable because infected specimens of some species can be selectively collected based on depigmentation of mouthparts.
一种可导致无尾目动物发病和死亡的真菌病原体——蛙壶菌(Batrachochytrium dendrobatidis,简称Bd),已在全球范围内影响两栖动物种群。获得Bd分离株的纯培养物对于了解这种病原体生态学的实验研究至关重要。我们评估了丽斑雨蛙(Hylodes cf. ornatus)和牛蛙(Lithobates catesbeianus)蝌蚪的体长与口部色素脱失之间的关系,并确定角质层脱失是否表明感染了蛙壶菌。来自南美洲(巴西:圣保罗)和北美洲(美国:缅因州)的这两个物种之间存在很强的关联。我们认为,如果可以避免,不杀死成年两栖动物是谨慎的做法,因此从蝌蚪中获取用于分离Bd的组织是合理的,因为可以根据口部色素脱失情况选择性地收集某些物种的感染标本。