Omali Negar Babaei, Zhu Hua, Zhao Zhenjun, Willcox Mark D P
Brien Holden Vision Institute, School of Optometry and Vision Science, University of New South Wales, Sydney, New South Wales, Australia.
Optom Vis Sci. 2013 Jun;90(6):557-64. doi: 10.1097/OPX.0b013e318292bb13.
Bacterial adhesion to contact lenses is believed to be the initial step for the development of several adverse reactions that occur during lens wear such as microbial keratitis. This study examined the effect of combinations of proteins on the adhesion of bacteria to contact lenses.
Unworn balafilcon A and senofilcon A lenses were soaked in commercially available pure protein mixtures to achieve the same amount of various proteins as found ex vivo. These lenses were then exposed to Pseudomonas aeruginosa and Staphylococcus aureus. Following incubation, the numbers of P. aeruginosa or S. aureus that adhered to the lenses were measured. The possible effect of proteins on bacterial growth was investigated by incubating bacteria in medium containing protein.
Although there was a significant (p < 0.003) increase in the total or viable counts of one strain of S. aureus (031) on balafilcon A lenses soaked in the lysozyme/lactoferrin combination, the protein adhered to lenses did not alter the adhesion of any other strains of P. aeruginosa or S. aureus (p > 0.05). Growth of S. aureus 031 (p < 0.0001) but not of P. aeruginosa 6294 was stimulated by addition of lysozyme/lactoferrin combination (2.8/0.5 mg/mL). Addition of lipocalin did not affect the growth of any strains tested (p > 0.05).
Adsorption of amounts of lysozyme and lactoferrin or lipocalin equivalent to those extracted from worn contact lenses did not affect the adhesion of most strains of S. aureus or P. aeruginosa to lens surfaces.
细菌黏附于隐形眼镜被认为是佩戴隐形眼镜期间发生的几种不良反应（如微生物性角膜炎）发展的初始步骤。本研究考察了蛋白质组合对细菌黏附于隐形眼镜的影响。
将未佩戴过的百超菲康A和森菲康A隐形眼镜浸泡在市售纯蛋白质混合物中，以达到与体内发现的各种蛋白质相同的量。然后将这些隐形眼镜暴露于铜绿假单胞菌和金黄色葡萄球菌。孵育后，测量黏附于隐形眼镜上的铜绿假单胞菌或金黄色葡萄球菌的数量。通过在含蛋白质的培养基中孵育细菌来研究蛋白质对细菌生长的可能影响。
虽然浸泡在溶菌酶/乳铁蛋白组合中的百超菲康A隐形眼镜上的一株金黄色葡萄球菌（031）的总数或活菌数有显著增加（p < 0.003），但黏附于隐形眼镜上的蛋白质并未改变任何其他铜绿假单胞菌或金黄色葡萄球菌菌株的黏附情况（p > 0.05）。添加溶菌酶/乳铁蛋白组合（2.8/0.5 mg/mL）可刺激金黄色葡萄球菌031的生长（p < 0.0001），但对铜绿假单胞菌6294无影响。添加脂质运载蛋白对任何测试菌株的生长均无影响（p > 0.05）。
吸附相当于从佩戴过的隐形眼镜中提取的溶菌酶、乳铁蛋白或脂质运载蛋白的量，不会影响大多数金黄色葡萄球菌或铜绿假单胞菌菌株对镜片表面的黏附。
