Czech Collection of Microorganisms, Department of Experimental Biology, Faculty of Science, Masaryk University, Tvrdého 14, Brno, 60200, Czech Republic,
Folia Microbiol (Praha). 2013 Nov;58(6):649-56. doi: 10.1007/s12223-013-0254-6. Epub 2013 May 5.
Early childhood caries (ECC) has become a serious medical problem worldwide in the last decade. Bacterial microflora of the dental plaque and oral cavity is considered an important factor in the formation and progression of dental caries. The aim of this study was strain typing and comparison of bacterial isolates retrieved from caries lesions and root canal contents of the same teeth. In total, 18 pairs of presumptive streptococci and lactobacilli retrieved from dental caries and root canals isolated from ECC-affected children, were selected on the basis of biotyping results and rep-PCR fingerprinting with (GTG)5 primer. Strain typing was further done using the RiboPrinter microbial characterization system (DuPont Qualicon). The automated ribotyping determined 14 pairs of the strains (77.8 %) to be identical. The results obtained confirmed that identical bacterial strains colonized both the decayed dental surface and the necrotic content of the dental pulp cavity during the cariogenesis. Our finding supports the assumption that bacteria could penetrate through the damaged dental surface to the inner parts of the teeth.
在过去十年中，婴幼儿龋（ECC）已成为全球严重的医学问题。牙菌斑和口腔的细菌微生物群被认为是龋齿形成和进展的重要因素。本研究的目的是对从龋病病变和同一牙齿根管内容物中分离出的细菌进行菌株分型和比较。总共从受 ECC 影响的儿童的龋病和根管中分离出 18 对推定链球菌和乳杆菌，根据生物分型结果和（GTG）5 引物的重复序列扩增片段长度多态性（rep-PCR）指纹图谱进行选择。使用 RiboPrinter 微生物特征系统（DuPont Qualicon）进一步进行菌株分型。自动化核糖体分型确定了 14 对（77.8%）菌株相同。所得结果证实，在龋病发生过程中，相同的细菌菌株定植于受损的牙面和牙髓腔的坏死内容物上。我们的发现支持这样一种假设，即细菌可以穿透受损的牙面进入牙齿内部。
