肠球菌 Rgg 样调控因子 ElrR 激活 elrA 操纵子的表达。
Enterococcal Rgg-like regulator ElrR activates expression of the elrA operon.
机构信息
INRA, UMR1319 Micalis, Jouy-en-Josas, France.
出版信息
J Bacteriol. 2013 Jul;195(13):3073-83. doi: 10.1128/JB.00121-13. Epub 2013 May 3.
Abstract
The Enterococcus faecalis leucine-rich protein ElrA promotes virulence by stimulating bacterial persistence in macrophages and production of the interleukin-6 (IL-6) cytokine. The ElrA protein is encoded within an operon that is poorly expressed under laboratory conditions but induced in vivo. In this study, we identify ef2687 (renamed elrR), which encodes a member of the Rgg (regulator gene for glucosyltransferase) family of putative regulatory proteins. Using quantitative reverse transcription-PCR, translational lacZ fusions, and electrophoretic mobility shift assays, we demonstrate that ElrR positively regulates expression of elrA. These results correlate with the attenuated virulence of the ΔelrR strain in a mouse peritonitis model. Virulence of simple and double elrR and elrA deletion mutants also suggests a remaining ElrR-independent expression of elrA in vivo and additional virulence-related genes controlled by ElrR.
摘要
粪肠球菌富含亮氨酸蛋白 ElrA 通过刺激巨噬细胞中的细菌持续存在和白细胞介素-6 (IL-6) 细胞因子的产生来促进毒力。ElrA 蛋白编码在一个操纵子中，该操纵子在实验室条件下表达不佳，但在体内诱导。在这项研究中，我们鉴定了 ef2687（重命名为 elrR），它编码一个假定调节蛋白的 Rgg（葡糖基转移酶调节基因）家族的成员。使用定量逆转录-PCR、翻译 lacZ 融合和电泳迁移率变动分析，我们证明了 ElrR 正向调节 elrA 的表达。这些结果与ΔelrR 菌株在小鼠腹膜炎模型中减弱毒力的情况相关。简单和双 elrR 和 elrA 缺失突变体的毒力也表明体内仍存在 ElrR 不依赖的 elrA 表达，以及由 ElrR 控制的其他与毒力相关的基因。
相似文献
1
Enterococcal Rgg-like regulator ElrR activates expression of the elrA operon.肠球菌 Rgg 样调控因子 ElrR 激活 elrA 操纵子的表达。
J Bacteriol. 2013 Jul;195(13):3073-83. doi: 10.1128/JB.00121-13. Epub 2013 May 3.
2
Overexpression of Enterococcus faecalis elr operon protects from phagocytosis.粪肠球菌elr操纵子的过表达可保护其免受吞噬作用。
BMC Microbiol. 2015 May 25;15:112. doi: 10.1186/s12866-015-0448-y.
3
Enterococcal leucine-rich repeat-containing protein involved in virulence and host inflammatory response.参与毒力和宿主炎症反应的含肠球菌富含亮氨酸重复序列蛋白
Infect Immun. 2007 Sep;75(9):4463-71. doi: 10.1128/IAI.00279-07. Epub 2007 Jul 9.
4
Exploration of the role of the virulence factor ElrA during Enterococcus faecalis cell infection.探索毒力因子 ElrA 在粪肠球菌细胞感染过程中的作用。
Sci Rep. 2018 Jan 29;8(1):1749. doi: 10.1038/s41598-018-20206-6.
5
Characterization of the Ers regulon of Enterococcus faecalis.粪肠球菌Ers调控子的表征
Infect Immun. 2008 Jul;76(7):3064-74. doi: 10.1128/IAI.00161-08. Epub 2008 Apr 21.
6
Manganese-dependent regulation of the endocarditis-associated virulence factor EfaA of Enterococcus faecalis.粪肠球菌心内膜炎相关毒力因子EfaA的锰依赖性调控
J Med Microbiol. 2003 Feb;52(Pt 2):113-119. doi: 10.1099/jmm.0.05039-0.
7
EfaR is a major regulator of Enterococcus faecalis manganese transporters and influences processes involved in host colonization and infection.EfaR 是粪肠球菌锰转运蛋白的主要调控因子，影响宿主定植和感染相关的过程。
Infect Immun. 2013 Mar;81(3):935-44. doi: 10.1128/IAI.06377-11. Epub 2013 Jan 7.
8
Enterococcal cytolysin: activities and association with other virulence traits in a pathogenicity island.肠球菌溶血素：致病岛中的活性及其与其他毒力特性的关联
Int J Med Microbiol. 2004 Apr;293(7-8):609-18. doi: 10.1078/1438-4221-00301.
9
Enterococcus faecalis virulence regulator FsrA binding to target promoters.粪肠球菌毒力调节因子 FsrA 与靶启动子的结合。
J Bacteriol. 2011 Apr;193(7):1527-32. doi: 10.1128/JB.01522-10. Epub 2011 Jan 21.
10
The Enterococcus faecalis virulence factor ElrA interacts with the human Four-and-a-Half LIM Domains Protein 2.粪肠球菌毒力因子 ElrA 与人的 Four-and-a-Half LIM Domains 蛋白 2 相互作用。
Sci Rep. 2017 Jul 4;7(1):4581. doi: 10.1038/s41598-017-04875-3.
引用本文的文献
1
Detecting co-selection through excess linkage disequilibrium in bacterial genomes.通过细菌基因组中过度的连锁不平衡检测共选择。
NAR Genom Bioinform. 2024 Jun 6;6(2):lqae061. doi: 10.1093/nargab/lqae061. eCollection 2024 Jun.
2
Draft Genome Sequences of Two Commensal Enterococcus faecalis Strains Isolated from American Black Vultures (Coragyps atratus) in Brazil.从巴西美洲黑兀鹫（黑美洲鹫）中分离出的两株粪肠球菌共生菌株的基因组序列草图
Microbiol Resour Announc. 2022 Aug 18;11(8):e0005722. doi: 10.1128/mra.00057-22. Epub 2022 Jul 11.
3
Involvement of Chromosomally Encoded Homologs of the RRNPP Protein Family in Enterococcus faecalis Biofilm Formation and Urinary Tract Infection Pathogenesis.染色体编码的 RRNPP 蛋白家族同源物参与粪肠球菌生物膜形成和尿路感染发病机制。
J Bacteriol. 2020 Aug 10;202(17). doi: 10.1128/JB.00063-20.
4
Production of Membrane Vesicles by Cultured With or Without Subinhibitory Concentrations of Antibiotics and Their Pathological Effects on Epithelial Cells.培养物中添加或不添加亚抑菌浓度抗生素对膜泡的产生及其对上皮细胞的病理影响。
Front Cell Infect Microbiol. 2019 Aug 14;9:295. doi: 10.3389/fcimb.2019.00295. eCollection 2019.
5
Countermeasures Defeat a Virulent Bacteriophage.抗击烈性噬菌体的对策。
Viruses. 2019 Jan 10;11(1):48. doi: 10.3390/v11010048.
6
Exploration of the role of the virulence factor ElrA during Enterococcus faecalis cell infection.探索毒力因子 ElrA 在粪肠球菌细胞感染过程中的作用。
Sci Rep. 2018 Jan 29;8(1):1749. doi: 10.1038/s41598-018-20206-6.
7
Model systems for the study of Enterococcal colonization and infection.肠球菌定植和感染的模型系统。
Virulence. 2017 Nov 17;8(8):1525-1562. doi: 10.1080/21505594.2017.1279766. Epub 2017 May 4.
8
Overexpression of Enterococcus faecalis elr operon protects from phagocytosis.粪肠球菌elr操纵子的过表达可保护其免受吞噬作用。
BMC Microbiol. 2015 May 25;15:112. doi: 10.1186/s12866-015-0448-y.
9
An ABC transporter is required for secretion of peptide sex pheromones in Enterococcus faecalis.粪肠球菌分泌肽类性信息素需要一种ABC转运蛋白。
mBio. 2014 Sep 23;5(5):e01726-14. doi: 10.1128/mBio.01726-14.
10
Peptide pheromone signaling in Streptococcus and Enterococcus.链球菌和肠球菌中的肽信息素信号传导。
FEMS Microbiol Rev. 2014 May;38(3):473-92. doi: 10.1111/1574-6976.12046. Epub 2013 Oct 31.
本文引用的文献
1
Structural basis for the activation mechanism of the PlcR virulence regulator by the quorum-sensing signal peptide PapR.PlcR 毒力调节剂被群体感应信号肽 PapR 激活的结构基础。
Proc Natl Acad Sci U S A. 2013 Jan 15;110(3):1047-52. doi: 10.1073/pnas.1213770110. Epub 2012 Dec 31.
2
Antagonistic Rgg regulators mediate quorum sensing via competitive DNA binding in Streptococcus pyogenes.抗 Rgg 调节蛋白通过与 DNA 的竞争结合介导酿脓链球菌中的群体感应。
mBio. 2013 Jan 2;3(6):e00333-12. doi: 10.1128/mBio.00333-12.
3
Direct substitution and assisted dissociation pathways for turning off transcription by a MerR-family metalloregulator.MerR 家族金属调控因子转录失活的直接取代和辅助解离途径。
Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15121-6. doi: 10.1073/pnas.1208508109. Epub 2012 Sep 4.
4
In vivo and in vitro analyses of regulation of the pheromone-responsive prgQ promoter by the PrgX pheromone receptor protein.体内和体外分析 PrgX 信息素受体蛋白对信息素响应的 prgQ 启动子的调控。
J Bacteriol. 2012 Jul;194(13):3386-94. doi: 10.1128/JB.00364-12. Epub 2012 Apr 27.
5
Large-scale screening of a targeted Enterococcus faecalis mutant library identifies envelope fitness factors.大规模筛选靶向粪肠球菌突变文库鉴定包膜适应因子。
PLoS One. 2011;6(12):e29023. doi: 10.1371/journal.pone.0029023. Epub 2011 Dec 15.
6
An amino-terminal signal peptide of Vfr protein negatively influences RopB-dependent SpeB expression and attenuates virulence in Streptococcus pyogenes.Vfr 蛋白的氨基端信号肽负调控 RopB 依赖性 SpeB 表达并减弱酿脓链球菌的毒力。
Mol Microbiol. 2011 Dec;82(6):1481-95. doi: 10.1111/j.1365-2958.2011.07902.x. Epub 2011 Nov 21.
7
Two group A streptococcal peptide pheromones act through opposing Rgg regulators to control biofilm development.两组 A 族链球菌肽信息素通过相反的 Rgg 调节剂作用控制生物膜的发展。
PLoS Pathog. 2011 Aug;7(8):e1002190. doi: 10.1371/journal.ppat.1002190. Epub 2011 Aug 4.
8
Identification of Rgg binding sites in the Streptococcus pyogenes chromosome.鉴定酿脓链球菌染色体上的 Rgg 结合位点。
J Bacteriol. 2011 Sep;193(18):4933-42. doi: 10.1128/JB.00429-11. Epub 2011 Jul 15.
9
Naturally occurring single amino acid replacements in a regulatory protein alter streptococcal gene expression and virulence in mice.天然存在的调节蛋白中的单个氨基酸替换会改变链球菌在小鼠中的基因表达和毒力。
J Clin Invest. 2011 May;121(5):1956-68. doi: 10.1172/JCI45169. Epub 2011 Apr 1.
10
Rgg proteins associated with internalized small hydrophobic peptides: a new quorum-sensing mechanism in streptococci.与内化的小疏水性肽相关的 Rgg 蛋白：链球菌中的一种新的群体感应机制。
Mol Microbiol. 2011 May;80(4):1102-19. doi: 10.1111/j.1365-2958.2011.07633.x. Epub 2011 Mar 30.
Zotero 插件