Department of Surgery, County Hospital PoŽega, Osječka 107, HR-34 000 PoŽega, Croatia.
Anticancer Res. 2013 May;33(5):2043-6.
In the present work, we compared the antitumor effects of native human interferon-α (IFN-α) (nHuIFN-α) and recombinant human IFN-α (rHuIFN-α) on human lung adenocarcinoma A549 cells.
The antitumor activity was determined by measuring cell viability and apoptosis, while the abundance of mRNA, measured by polymerase chain reaction (PCR), determined the potential role of p21 and survivin in antitumor activity of nHuIFN-α.
The results show that nHuIFN-α significantly reduced A549 cell viability, compared to rHuIFN-α. The most potent effect of nHuIFN-α was also observed when apoptosis was measured. A549 cells treated with nHuIFN-α expressed a significantly higher amount of p21 mRNA, while the amount of survivin mRNA was significantly reduced.
Considering both the anti-proliferative and anti-apoptotic effects of each IFN-α, we conclude that further elucidation of the mechanisms of the antitumor activity of nHuIFN-α will help in producing more effective and less toxic therapeutic protocols and preparations.
在本工作中，我们比较了天然人干扰素-α（IFN-α）（nHuIFN-α）和重组人干扰素-α（rHuIFN-α）对人肺腺癌 A549 细胞的抗肿瘤作用。
通过测量细胞活力和细胞凋亡来确定抗肿瘤活性，而聚合酶链反应（PCR）测量的 mRNA 丰度则确定了 p21 和 survivin 在 nHuIFN-α抗肿瘤活性中的潜在作用。
结果表明，与 rHuIFN-α相比，nHuIFN-α显著降低了 A549 细胞活力。当测量细胞凋亡时，nHuIFN-α也观察到了最有效的作用。用 nHuIFN-α处理的 A549 细胞表达了明显更高水平的 p21 mRNA，而 survivin mRNA 的量则明显降低。
考虑到每种 IFN-α 的抗增殖和抗凋亡作用，我们得出结论，进一步阐明 nHuIFN-α抗肿瘤活性的机制将有助于产生更有效和毒性更小的治疗方案和制剂。
