Liu Chunxiu, Song Yilin, Lin Nansen, Zhou Shuai, Wang Mixia, Cai Xinxia
State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China.
J Nanosci Nanotechnol. 2013 Feb;13(2):736-40. doi: 10.1166/jnn.2013.6015.
The microfabricated microelectrode arrays on glass substrate combined with homemade multichannel detection system as a powerful tool for synchronous neurochemical and neuroelectrial monitoring was described. The dual-mode (neurochemical and neuroelectrial: simultaneous recording of spikes, and dopamine overflow on the same spatiotemporal scale) recording was carried in PBS buffer. The neurochemical constant potential amperometry was carried at +0.4 V working potential for nM-microM dopamine detection and nM level dopamine concentration increase can be detected by the device. The microelectrode chip showed high sensitivity of 0.88 nA/nM mm2 during nM range and 0.43 nA/nM mm2 during microM range for the determination of DA. The amperometric method used to detect dopamine (DA) did not significantly influence electrophysiological activity at low concentration (< 200 nM) and have a quickly clear away strike at high concentration (> 2 microM). The system uniquely affords synchronous measurement of chemical and electrical neural activity at the adjacent recording sites on the same chip. The sensitivity and resolution of the device allows for simultaneous recoding of electrical and chemical signaling, which may be useful for examining specific spatiotemporal relationships between electrical and chemical signaling in vitro.
本文描述了一种基于玻璃基板的微纳加工微电极阵列与自制多通道检测系统相结合的技术，该技术作为一种强大的工具，可用于同步神经化学和神经电监测。在磷酸盐缓冲液（PBS）中进行了双模式（神经化学和神经电：在相同的时空尺度上同时记录尖峰和多巴胺溢出）记录。神经化学恒电位安培法在+0.4 V工作电位下进行，用于检测纳摩尔至微摩尔浓度的多巴胺，该装置能够检测出纳摩尔水平的多巴胺浓度增加。在检测多巴胺时，微电极芯片在纳摩尔浓度范围内对多巴胺的检测灵敏度为0.88 nA/nM·mm2，在微摩尔浓度范围内为0.43 nA/nM·mm2。用于检测多巴胺（DA）的安培法在低浓度（<200 nM）时对电生理活动没有显著影响，在高浓度（>2 microM）时具有快速清除作用。该系统独特地能够在同一芯片上的相邻记录位点同步测量化学和电神经活动。该装置的灵敏度和分辨率允许同时记录电信号和化学信号，这对于研究体外电信号和化学信号之间的特定时空关系可能是有用的。
